p73 beta inhibits transcriptional activities of enhancer I and X promoter in hepatitis B virus more efficiently than p73 alpha

	p73 beta inhibits transcriptional activities of enhancer I and X promoter in hepatitis B virus more efficiently than p73 alpha
	Xu, ZH; Zhao, MJ; Li, TP
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2002
卷号	8 期号:6 页码:1094-1097
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Mol Biol, PO Box 35,320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	AIM: p73, as a novel member of a family of p53-related transcription factors, shares redundant functions with p53, such as the abilities of inducing apoptosis and suppressing growth. It is well known that p53 can repress HBV expression and transcription efficiently. The aim of this paper is to investigate the transcriptional effect of p73alpha and p73beta on hepatitis B virus (HBV) and to understand the correlation between HBV and p73. METHODS: To construct an x-gene inactivated HBV plasmid which was cotransfected with p73alpha or p73beta expression vectors into HepG2 cells. After transiently transfection, HBV surface antigen (HBsAg) and HBV e antigen (HBeAg) were detected by ELISA. Viral transcripts synthesized by HBV were evaluated by Northern blotting analysis. The activities of HBV regulatory elements, including enhancer I/X promoter (ENI/Xp) and enhancer II/core promoter (ENII/Cp) were monitored by luciferase assays. RESULTS: Both p73alpha and p73beta could repress HBsAg and HBeAg expression by downregulating the ENI/Xp and ENII/ Cp activities. But p73beta exerted stronger inhibition on the activity of ENI/Xp than p73(x, resulting in much lower level of viral transcripts and the antigens expression. CONCLUSION: p73beta as a novel member of p53 family can efficiently inhibit HBV transcription mainly through downregulating the activities of the HBV ENI/Xp regulatory elements.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
关键词[WOS]	HEPATOCELLULAR-CARCINOMA ; P73 GENE ; CHROMOSOME 1P36.3 ; P53 ; MUTATION ; EXPRESSION ; NEUROBLASTOMA ; REPLICATION ; ABSENCE ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000179741700027
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2517]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, ZH,Zhao, MJ,Li, TP. p73 beta inhibits transcriptional activities of enhancer I and X promoter in hepatitis B virus more efficiently than p73 alpha[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2002,8(6):1094-1097.
APA	Xu, ZH,Zhao, MJ,&Li, TP.(2002).p73 beta inhibits transcriptional activities of enhancer I and X promoter in hepatitis B virus more efficiently than p73 alpha.WORLD JOURNAL OF GASTROENTEROLOGY,8(6),1094-1097.
MLA	Xu, ZH,et al."p73 beta inhibits transcriptional activities of enhancer I and X promoter in hepatitis B virus more efficiently than p73 alpha".WORLD JOURNAL OF GASTROENTEROLOGY 8.6(2002):1094-1097.