Protein isoforms observed by ultrahigh resolution capillary isoelectric focusing-electrospray ionization mass spectrometry

	Protein isoforms observed by ultrahigh resolution capillary isoelectric focusing-electrospray ionization mass spectrometry
	Liu, T; Shao, XX; Zeng, R; Xia, QC
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2002
卷号	34 期号:4 页码:423-432
关键词	capillary isoelectric focusing electrospray ionization mass spectrometry protein isoform hemoglobin variants
通讯作者	,
英文摘要	On-line coupling of capillary isoelectric focusing (CIEF) to electrospray ionization mass spectrometry (ESI-MS) as a two-dimensional separation/analysis system was employed for high-resolution analysis of the protein isoforms observed during CIEF process. The analytical system was established by using neutral coated long capillary (80 cm), active capillary positioning and sheath-liquid interface. Proteins were separated and resolved in CIEF according to their differences in isoelectric point (pI), and then characterized by ESI-NIS. The focused protein zones were eluted to the entrance of MS by combining cathodic mobilization with gravity. The ultrahigh resolution (difference in pI < 0.04) of this technique obtained under certain conditions led to the detection of three isoforms in hemoglobin A and in sickle cell hemoglobin (with similar charge distribution and same molecular weight, but their differences in pI ranging from 0. 04 to 0. 08) and two isoforms of P-lactoglobulin A (difference in pI is 0. 6). The isoelectric points, relative amounts, and molecular masses of these isoforms were determined simultaneously by CIEF-ESI-MS.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	ELECTROPHORESIS ; DISSOCIATION ; TRANSFERRIN ; INTERFACE ; MIXTURES
收录类别	SCI
语种	英语
WOS记录号	WOS:000177017300007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2480]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, T,Shao, XX,Zeng, R,et al. Protein isoforms observed by ultrahigh resolution capillary isoelectric focusing-electrospray ionization mass spectrometry[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(4):423-432.
APA	Liu, T,Shao, XX,Zeng, R,&Xia, QC.(2002).Protein isoforms observed by ultrahigh resolution capillary isoelectric focusing-electrospray ionization mass spectrometry.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(4),423-432.
MLA	Liu, T,et al."Protein isoforms observed by ultrahigh resolution capillary isoelectric focusing-electrospray ionization mass spectrometry".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.4(2002):423-432.