Activation mechanism of solubilized epidermal growth factor receptor tyrosine kinase

	Activation mechanism of solubilized epidermal growth factor receptor tyrosine kinase
	Ge, GX; Wu, J; Wang, Y; Lin, QS
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2002
卷号	290 期号:3 页码:914-920
关键词	epidermal growth factor (EGF) EGF receptor receptor tyrosine kinase activation mechanism
通讯作者	Lin, QS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Dimerization of epidermal growth factor receptor (EGFR) leads to the activation of its tyrosine kinase. To elucidate whether dimerization is responsible for activation of the intracellular tyrosine kinase domain or just plays a role in the stabilization of the active form, the activated status of wild-type EGFR moiety in the heterodimer with kinase activity-deficient mutant receptors was investigated. The kinase activity of the wildtype EGFR was partially activated by EGF in the heterodimer with intracellular domain deletion (sEGFR) or ATP binding-deficient mutant (K721A) EGFRs, while the wild-type EGFR in the heterodimer of wild-type and phosphate transfer activity-deficient mutant receptor D813N could be fully activated. After treatment with EGF, the ATP binding affinity and the V-max of the wildtype EGFR increased. In the presence of sEGFR, a similar increase in the affinity for ATP was observed, but V-max did not change. A two-step activation mechanism for EGFR was proposed: upon binding of EGF, the affin -ity for ATP increased and then, as a result of interaction between the neighboring tyrosine kinase domain, V-max increased. (C) 2002 Elsevier Science (USA).
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	FACTOR EGF RECEPTOR ; SELF-PHOSPHORYLATION ; BINDING-SITE ; DIMERIZATION ; AUTOPHOSPHORYLATION ; MUTANTS ; MODEL ; MUTATION ; AFFINITY ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000173611500006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2473]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ge, GX,Wu, J,Wang, Y,et al. Activation mechanism of solubilized epidermal growth factor receptor tyrosine kinase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,290(3):914-920.
APA	Ge, GX,Wu, J,Wang, Y,&Lin, QS.(2002).Activation mechanism of solubilized epidermal growth factor receptor tyrosine kinase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,290(3),914-920.
MLA	Ge, GX,et al."Activation mechanism of solubilized epidermal growth factor receptor tyrosine kinase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 290.3(2002):914-920.