Get effective polyclonal antisera in one month

	Get effective polyclonal antisera in one month
	Hu, YX; Guo, JY; Shen, L; Chen, Y; Zhang, ZC; Zhang, YL
刊名	CELL RESEARCH
	2002
卷号	12 期号:2 页码:157-160
关键词	polyclonal antisera preparation modified immunization method
通讯作者	Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general, high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4 months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis. It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	SUPERFAMILY
收录类别	SCI
语种	英语
WOS记录号	WOS:000176627300009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2436]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, YX,Guo, JY,Shen, L,et al. Get effective polyclonal antisera in one month[J]. CELL RESEARCH,2002,12(2):157-160.
APA	Hu, YX,Guo, JY,Shen, L,Chen, Y,Zhang, ZC,&Zhang, YL.(2002).Get effective polyclonal antisera in one month.CELL RESEARCH,12(2),157-160.
MLA	Hu, YX,et al."Get effective polyclonal antisera in one month".CELL RESEARCH 12.2(2002):157-160.