Expression of toll-like receptors 2 and 4 and CD14 during differentiation of HL-60 cells induced by phorbol 12-myristate 13-acetate and 1 alpha, 25-dihydroxy-vitamin D-3

	Expression of toll-like receptors 2 and 4 and CD14 during differentiation of HL-60 cells induced by phorbol 12-myristate 13-acetate and 1 alpha, 25-dihydroxy-vitamin D-3
	Li, CL; Wang, YB; Gao, L; Zhang, JS; Shao, J; Wang, SN; Feng, WG; Wang, XY; Li, ML; Chang, ZL
刊名	CELL GROWTH & DIFFERENTIATION
	2002
卷号	13 期号:1 页码:27-38
通讯作者	Chang, ZL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Immune Cells Signaling, Room 1104,Cell Biol Bldg,320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Macrophages form a crucial bridge between the innate and adaptive immune response. One of their most important functions is to recognize infectious microorganisms. Toll-like receptors (TLRs) are key elements in pathogen recognition, and among them, TLR2 and TLR4 are most discussed. However, expression patterns of TLRs during myeloid cell differentiation to macrophage are unknown. In this study, we examined differentiation in the model human myeloid cell line, HL-60, treated with phorbol 12-myristate 13-acetate (PMA) or VitD(3). Expression of TLR2, TLR4, and CD14 were measured by reverse transcription-PCR, RNase protection assay, and fluorescence-activated cell sorter assays. After treatment by PMA (1, 10, and 100 nm) for 12, 24, and 48 h, expression of TLR2 and CD14 mRNA was increased in a time- and dose-dependent manner. However, VitD(3) only induced expression of CD14 but not TLR2 in HL-60 cells. TLR4 was expressed constitutively before differentiation and increased slightly after that. Thus, PMA-mediated differentiation of HL-60 cells to macrophages is associated largely with TLR2 expression and, to a much lesser extent, with TLR4. Furthermore, up-regulation of TLR2 and CD14 mRNA expression by PMA was abrogated by a protein kinase C inhibitor, Calphostine C, suggesting the up-regulation of TLR2 and CD14 mRNA is dependent on the activation of protein kinase C. Coexpression of CD14/TLR2 and/or CD14/TLR4 may be essential but not sufficient for the production of tumor necrosis factor-a in response to lipopolysaccharide in our system.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	PROMYELOCYTIC LEUKEMIA-CELLS ; PROTEIN-KINASE-C ; BACTERIAL LIPOPOLYSACCHARIDE ; GENE EXPRESSIONS ; DROSOPHILA TOLL ; KAPPA-B ; FAMILY ; MACROPHAGES ; ESCHERICHIA ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000173550700004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2428]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, CL,Wang, YB,Gao, L,et al. Expression of toll-like receptors 2 and 4 and CD14 during differentiation of HL-60 cells induced by phorbol 12-myristate 13-acetate and 1 alpha, 25-dihydroxy-vitamin D-3[J]. CELL GROWTH & DIFFERENTIATION,2002,13(1):27-38.
APA	Li, CL.,Wang, YB.,Gao, L.,Zhang, JS.,Shao, J.,...&Chang, ZL.(2002).Expression of toll-like receptors 2 and 4 and CD14 during differentiation of HL-60 cells induced by phorbol 12-myristate 13-acetate and 1 alpha, 25-dihydroxy-vitamin D-3.CELL GROWTH & DIFFERENTIATION,13(1),27-38.
MLA	Li, CL,et al."Expression of toll-like receptors 2 and 4 and CD14 during differentiation of HL-60 cells induced by phorbol 12-myristate 13-acetate and 1 alpha, 25-dihydroxy-vitamin D-3".CELL GROWTH & DIFFERENTIATION 13.1(2002):27-38.