N-desulfated heparin improves concanavalin A-induced liver injury partly through inhibiting T lymphocyte adhesion

	N-desulfated heparin improves concanavalin A-induced liver injury partly through inhibiting T lymphocyte adhesion
	Yuan, L; Geng, JG; Xu, Q
刊名	INFLAMMATION RESEARCH
	2003
卷号	52 期号:9 页码:383-389
关键词	N-desulfated heparin Con A liver injury lymphocytes adhesion
通讯作者	Xu, Q (reprint author), Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, 22 Han Kou Rd, Nanjing 210093, Peoples R China.,
英文摘要	Objective and Design: To examine the effect of N-desulfated heparin on Concanavalin A (Con A)-induced liver injury and its mechanisms of action. Materials and methods: Liver injury was induced in mice by Con A. The in vitro assays for examining the adhesions of spleen T lymphocytes and Jurkat cells to extracellular matrix (ECM) were also performed. Results: N-desulfated heparin significantly inhibited the elevation in alanine transaminase, aspartate transaminase and lactic dehydrogenase activities in serum, and recovered the superoxide dismutase activity in the liver tissue of mice with liver injury. In liver histological examination, the inflammatory infiltration, hepatocyte degeneration and Kupffer cell hyperplasia were remarkably improved by N-desulfated heparin. Multiple administrations of the heparin derivative for one day showed a more potent prevention of the liver injury than did single dosing for three days. N-desulfated heparin significantly inhibited the adhesion of spleen cells and purified T lymphocytes isolated from the liver injured mice to either type I collagen or fibronectin but not to laminin. The heparin derivative also showed a similar inhibition of the adhesion of spleen cells from normal mice, stimulated in vitro with Con A, whereas it did not affect their proliferation. Moreover, the adhesion of human leukemia Jurkat cells to collagen I was inhibited by N-desulfated heparin. Conclusion: N-desulfated heparin may improve immunological liver injury partly via reducing the functions, such as the adhesion to ECM, of T lymphocytes.
学科主题	Cell Biology; Immunology
类目[WOS]	Cell Biology ; Immunology
关键词[WOS]	NON-ANTICOAGULANT HEPARIN ; EXTRACELLULAR-MATRIX ; REPERFUSION INJURY ; IN-VIVO ; CELL ADHESION ; L-SELECTIN ; SULFATE ; MICE ; GLYCOSAMINOGLYCANS ; DYSFUNCTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000185481400006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2348]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yuan, L,Geng, JG,Xu, Q. N-desulfated heparin improves concanavalin A-induced liver injury partly through inhibiting T lymphocyte adhesion[J]. INFLAMMATION RESEARCH,2003,52(9):383-389.
APA	Yuan, L,Geng, JG,&Xu, Q.(2003).N-desulfated heparin improves concanavalin A-induced liver injury partly through inhibiting T lymphocyte adhesion.INFLAMMATION RESEARCH,52(9),383-389.
MLA	Yuan, L,et al."N-desulfated heparin improves concanavalin A-induced liver injury partly through inhibiting T lymphocyte adhesion".INFLAMMATION RESEARCH 52.9(2003):383-389.