Human alpha galactosidase and alpha 1,2 fucosyltransferase concordantly inhibit xenoreactivity of NIH 3T3 cells with human serum

	Human alpha galactosidase and alpha 1,2 fucosyltransferase concordantly inhibit xenoreactivity of NIH 3T3 cells with human serum
	Yan, JL; Yu, LY; Guo, LH
刊名	ACTA PHARMACOLOGICA SINICA
	2003
卷号	24 期号:9 页码:878-884
关键词	alpha-galactosidase alpha-L-fucosidase Gal alpha 1 NIH3T3 cell heterologous transplantation immunoglobulin M immunoglobulin G complement 3 Gal
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,lhguo@sunm.shcnc.ac.cn
英文摘要	AIM: To study the influence of the expression of human alpha galactosidase and alphal,2 fucosyltransferase on Gal alpha 1,3 Gal and consequent xenoreactivity in NIH3T3 cells. METHODS: The expression levels of G antigen and H antigen and binding of human natural antibodies (IgG and IgM) and complement (C3c) to NIH3T3 cells were analyzed by flow cytometry. Western blot was employed to further determine the expression of glycoproteins of G antigen. Cytolysis assay with normal human serum was performed by MTT assay. RESULTS: Western blot showed that glycoproteins with molecular weight of 107 kDa, 98 kDa, 88 kDa, 56 kDa, 40 kDa, and 37 kDa were inhibited and even abrogated totally in alpha galactosidase transfectants and alpha 1,2 fucosyltransferase transfectants. The combined transfection of the two enzymes led to a much stronger inhibition of the glycoproteins. The binding of G(S)-IB(4) was decreased by 57.4 % in alpha galactosidase transfectants, 28.8 % in alpha 1,2 fucosyltransferase transfectants, and 72.1 % in combined transfectants, respectively. In contrast, UEA-1 binding was increased about 6.7-fold, 6.0-fold, and 8.0-fold respectively. The xenoreactivity with human IgG was also reduced by 61.4 %, 67.0 %, and 73.4 %, respectively in the three kinds of transfectants. The resistance to cytolysis mediated by human serum was enhanced by 42.4 % in alpha galactosidase transfectants, 51.9 % in alpha 1,2 fucosyltranferase, and even 65.5 % in the combined transfectants. CONCLUSION: Although alpha galactosidase and alpha 1,2 fucosyltransferase had different biochemical properties, they could inhibit the expression of Gal alpha 1,3 Gal synergistically, leading to stronger resistance of xenograft against cytolysis.
学科主题	Chemistry; Pharmacology & Pharmacy
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
关键词[WOS]	ADENOVIRUS-MEDIATED EXPRESSION ; HYPERACUTE REJECTION ; ENDOTHELIAL-CELLS ; TARGET ANTIGENS ; ANTIBODIES ; PIG ; ALPHA-1,2-FUCOSYL-TRANSFERASE ; GAL-ALPHA(1,3)GAL ; CYTOTOXICITY ; XENOGRAFTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000185357500007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2345]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yan, JL,Yu, LY,Guo, LH. Human alpha galactosidase and alpha 1,2 fucosyltransferase concordantly inhibit xenoreactivity of NIH 3T3 cells with human serum[J]. ACTA PHARMACOLOGICA SINICA,2003,24(9):878-884.
APA	Yan, JL,Yu, LY,&Guo, LH.(2003).Human alpha galactosidase and alpha 1,2 fucosyltransferase concordantly inhibit xenoreactivity of NIH 3T3 cells with human serum.ACTA PHARMACOLOGICA SINICA,24(9),878-884.
MLA	Yan, JL,et al."Human alpha galactosidase and alpha 1,2 fucosyltransferase concordantly inhibit xenoreactivity of NIH 3T3 cells with human serum".ACTA PHARMACOLOGICA SINICA 24.9(2003):878-884.