Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor expression in U937 foam cells

	Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor expression in U937 foam cells
	Yang, PY; Rui, YC; Jin, YX; Li, TJ; Qiu, Y; Zhang, L; Wang, JS
刊名	ACTA PHARMACOLOGICA SINICA
	2003
卷号	24 期号:6 页码:610-614
关键词	antisense oligonucleotides vascular endothelium endothelial growth factors enzyme-linked immunosorbent assay Northern blotting U937 cells foam cells
通讯作者	Rui, YC (reprint author), Second Mil Med Univ, Sch Pharm, Dept Pharmacol, Shanghai 200433, Peoples R China.,ruiyc@smmu.edu.cn
英文摘要	AIM: To study the expression of vascular endothelial growth factor (VEGF) induced by oxidized low density liprotein (ox-LDL) and the inhibitory effects of antisense oligodeoxynucleotide (asODN) on the levels of VEGF protein and mRNA in the U937 foam cells. METHODS: U937 cells were incubated with ox-LDL 80 mg/L for 48 h, then, the foam cells were treated with asODN (0, 5, 10, and 20 mumol/L). The VEGF concentration in the media was determined by ELISA. The VEGF protein expression level in cells was measured by immuohistochemistry; the positive ratio detected by a morphometrical analysis system was used as the amount of the VEGF expression level. The VEGF mRNA level was examined by Northern blotting. RESULTS: After U937 cells were incubated with ox-LDL, VEGF expression level increased greatly both in the cells and in the media. asODN markedly inhibited the increase of VEGF. After treatment with asODN 20 mumol/L, the VEGF protein concentration in the media decreased by 45.0 %, the VEGF positive ratio detected by immuohistochemistry in cells decreased by 64.9 %, and the VEGF mRNA level decreased by 47.1 %. CONCLUSION: The expression of VEGF in U937 foam cells was strong. asODN inhibited VEGF expression significantly in U937 foam cells in vitro.
学科主题	Chemistry; Pharmacology & Pharmacy
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
关键词[WOS]	LOW-DENSITY-LIPOPROTEIN ; ANGIOGENESIS ; HYPERPERMEABILITY ; MACROPHAGES ; VEGF
收录类别	SCI
语种	英语
WOS记录号	WOS:000183575100020
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2318]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, PY,Rui, YC,Jin, YX,et al. Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor expression in U937 foam cells[J]. ACTA PHARMACOLOGICA SINICA,2003,24(6):610-614.
APA	Yang, PY.,Rui, YC.,Jin, YX.,Li, TJ.,Qiu, Y.,...&Wang, JS.(2003).Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor expression in U937 foam cells.ACTA PHARMACOLOGICA SINICA,24(6),610-614.
MLA	Yang, PY,et al."Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor expression in U937 foam cells".ACTA PHARMACOLOGICA SINICA 24.6(2003):610-614.