Mitosin/CENP-F is a conserved kinetochore protein subjected to cytoplasmic dynein-mediated poleward transport

	Mitosin/CENP-F is a conserved kinetochore protein subjected to cytoplasmic dynein-mediated poleward transport
	Yang, ZY; Guo, J; Li, N; Qian, M; Wang, SN; Zhu, XL
刊名	CELL RESEARCH
	2003
卷号	13 期号:4 页码:275-283
关键词	mitosin LEK1 CENP-F kinetochore
通讯作者	Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Mitosin/CENP-F is a human nuclear protein transiently associated with the outer kinetochore plate in M phase and is involved in M phase progression. LEK1 and CMF1, which are its murine and chicken orthologs, however, are implicated in muscle differentiation and reportedly not distributed at kinetochores. We therefore conducted several assays to clarify this issue. The typical centromere staining patterns were observed in mitotic cells from both human primary culture and murine., canine, and mink cell lines. A C-terminal portion of LEK1 also conferred centromere localization. Our analysis further suggests conserved kinetochore localization of mammalian mitosin orthologs. Moreover, mitosin was associated preferentially with kinetochores of unaligned chromosomes. It was also constantly transported from kinetochores to spindle poles by cytoplasmic dynein. These properties resemble those of other kinetochore proteins important for the spindle checkpoint., thus implying a role of mitosin in this checkpoint. Therefore, mitosin family may serve as multifunctional proteins involved in both mitosis and differentiation.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	CENP-F ; SPINDLE CHECKPOINT ; MITOTIC SPINDLE ; LOCALIZATION ; FARNESYLATION ; DIMERIZATION ; PROGRESSION ; COMPONENT ; TERMINUS ; DYNAMICS
收录类别	SCI
语种	英语
WOS记录号	WOS:000185166200007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2276]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, ZY,Guo, J,Li, N,et al. Mitosin/CENP-F is a conserved kinetochore protein subjected to cytoplasmic dynein-mediated poleward transport[J]. CELL RESEARCH,2003,13(4):275-283.
APA	Yang, ZY,Guo, J,Li, N,Qian, M,Wang, SN,&Zhu, XL.(2003).Mitosin/CENP-F is a conserved kinetochore protein subjected to cytoplasmic dynein-mediated poleward transport.CELL RESEARCH,13(4),275-283.
MLA	Yang, ZY,et al."Mitosin/CENP-F is a conserved kinetochore protein subjected to cytoplasmic dynein-mediated poleward transport".CELL RESEARCH 13.4(2003):275-283.