Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56

	Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56
	He, XB; Yan, YC; Li, YP; Koide, SS
刊名	CELL RESEARCH
	2003
卷号	13 期号:2 页码:121-129
关键词	ZP3 receptor sp56 rat testis sperm cloning
通讯作者	Yan, YC (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,
英文摘要	Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig, namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of 743 by and 938 by were amplified. The PCR products show very high homology to mouse sp56. However, the 743 by product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743 by product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that asimilar to2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr similar to42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. Rat sp56 was localized on rat sperm head by the indirect immunofluorescence method. Rat sp56 immunoreactivity was detected from the early pachytene spermatocytes and throughout the spermatogenesis. Its cloning will further our understanding of the mechanism of the sperm-egg recognition and binding.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	PROTEIN SP56 ; ACROSOMAL MATRIX ; EGG RECOGNITION ; FERTILIZATION ; IDENTIFICATION ; COMPONENT ; BINDING
收录类别	SCI
语种	英语
WOS记录号	WOS:000182632200005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2249]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	He, XB,Yan, YC,Li, YP,et al. Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56[J]. CELL RESEARCH,2003,13(2):121-129.
APA	He, XB,Yan, YC,Li, YP,&Koide, SS.(2003).Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56.CELL RESEARCH,13(2),121-129.
MLA	He, XB,et al."Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56".CELL RESEARCH 13.2(2003):121-129.