Integrated lectin affinity microfluidic chip for glycoform separation

	Integrated lectin affinity microfluidic chip for glycoform separation
	Mao, XL; Luo, Y; Dai, ZP; Wang, KY; Du, YG; Lin, BC
刊名	ANALYTICAL CHEMISTRY
	2004
卷号	76 期号:23 页码:6941-6947
通讯作者	Lin, BC (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, Beijing 100864, Peoples R China.,bclin@dicp.ac.cn
英文摘要	Lectin affinity chromatography was miniaturized into a microfluidic format, which results in improvement of performance, as compared to the conventional method. A lectin affinity monolith column was prepared in the microchannel of a microfluidic chip. The porous monolith was fabricated by UV-initiated polymerization of ethylene dimethacrylate (EDMA) and glycidyl methacrylate (GMA) in the presence of porogeneities, followed by immobilization of pisum sativum agglutinin (PSA) on the monolith matrix. Using electroosmosis as the driven force, lectin affinity chromatographies of three kinds of glycoprotein, turkey ovalbumin (TO), chicken ovalbumin (CO), and ovomucoid (OM), were carried out on the microfluidic system. All the glycoproteins were successfully separated into several fractions with different affinities toward the immobilized PSA. The integrated system reduces the time required for the lectin affinity chromatography reaction to similar to3%, thus, the overall analysis time from 4 h to 400 s. Only 300 pg of glycoprotein is required for the whole separation process. Moreover, troublesome operations for lectin affinity chromatography are simplified.
学科主题	Chemistry
类目[WOS]	Chemistry, Analytical
关键词[WOS]	LINKED SUGAR CHAINS ; CAPILLARY-ELECTROPHORESIS ; GLYCOPROTEIN GLYCOFORMS ; LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; BEADS ; CARCINOMA ; DIGESTION ; CHANNELS
收录类别	SCI
语种	英语
WOS记录号	WOS:000225535300021
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2179]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Mao, XL,Luo, Y,Dai, ZP,et al. Integrated lectin affinity microfluidic chip for glycoform separation[J]. ANALYTICAL CHEMISTRY,2004,76(23):6941-6947.
APA	Mao, XL,Luo, Y,Dai, ZP,Wang, KY,Du, YG,&Lin, BC.(2004).Integrated lectin affinity microfluidic chip for glycoform separation.ANALYTICAL CHEMISTRY,76(23),6941-6947.
MLA	Mao, XL,et al."Integrated lectin affinity microfluidic chip for glycoform separation".ANALYTICAL CHEMISTRY 76.23(2004):6941-6947.