Functional contribution of EEN to leukemogenic transformation by MLL-EEN fusion protein

	Functional contribution of EEN to leukemogenic transformation by MLL-EEN fusion protein
	Liu, H; Chen, B; Xiong, H; Huang, QH; Zhang, QH; Wang, ZG; Li, BL; Chen, Z; Chen, SJ
刊名	ONCOGENE
	2004
卷号	23 期号:19 页码:3385-3394
关键词	MLL-EEN transcriptional factor HoxA7 leukemogenesis
通讯作者	Chen, SJ (reprint author), Shanghai Med Univ 2, Ruijin Hosp, Shanghai Inst Hematol, State Key Lab Med Gen, 197 Ruijin Rd 2, Shanghai 200025, Peoples R China.,sjchen@stn.sh.cn
英文摘要	The EEN (extra eleven nineteen) gene was originally cloned from a case of acute myeloid leukemia M5 subtype with translocation t (11;19)(q23; p13), in which EEN was fused with MLL. To explore the involvement of EEN in leukemogenesis caused by MLL-EEN, we studied the transformation potential of the MLL-EEN fusion protein. MLL-EEN had oncogenic features, while, as a control, MLLDelta, the truncated form of MLL lacking the EEN moiety, did not show any oncogenic potential. MLL-EEN exerted a dominant-negative effect over wild-type EEN in terms of subcellular localization. Normally, EEN was found in the cytoplasm, but the MLL-EEN fusion protein was located in the nucleus, and EEN could be delocalized by MLL-EEN. This interaction is via a coiled-coil dimerization domain of EEN, which is reserved in the fusion protein. In addition, MLL-EEN might act as a potential transcriptional factor with the MLL part providing the DNA-binding domain and the EEN part providing the transcription activation domain, though EEN seems to have no direct role in transcriptional regulation. As an aberrant transcriptional factor, MLL-EEN could transactivate the promoter of HoxA7, a potential target gene of MLL.
学科主题	Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity
类目[WOS]	Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]	LEUKEMIA-CELL LINE ; SYNAPTIC VESICLE ENDOCYTOSIS ; CENTRAL-NERVOUS-SYSTEM ; HOMOLOGY 3 DOMAIN ; MYELOID PROGENITORS ; DOWN-REGULATION ; ANTISENSE OLIGODEOXYRIBONUCLEOTIDE ; CHROMOSOMAL TRANSLOCATION ; ONCOGENIC ACTIVATION ; BINDING PARTNERS
收录类别	SCI
语种	英语
WOS记录号	WOS:000220975000013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2172]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, H,Chen, B,Xiong, H,et al. Functional contribution of EEN to leukemogenic transformation by MLL-EEN fusion protein[J]. ONCOGENE,2004,23(19):3385-3394.
APA	Liu, H.,Chen, B.,Xiong, H.,Huang, QH.,Zhang, QH.,...&Chen, SJ.(2004).Functional contribution of EEN to leukemogenic transformation by MLL-EEN fusion protein.ONCOGENE,23(19),3385-3394.
MLA	Liu, H,et al."Functional contribution of EEN to leukemogenic transformation by MLL-EEN fusion protein".ONCOGENE 23.19(2004):3385-3394.