Effective gene-viral therapy for telomerase-positive cancers by selective replicative-competent adenovirus combining with endostatin gene

	Effective gene-viral therapy for telomerase-positive cancers by selective replicative-competent adenovirus combining with endostatin gene
	Zhang, Q; Nie, MM; Sham, J; Su, CQ; Xue, HB; Chua, D; Wang, WQ; Cui, ZF; Liu, YJ; Liu, C
刊名	CANCER RESEARCH
	2004
卷号	64 期号:15 页码:5390-5397
通讯作者	Qian, QJ (reprint author), Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Lab Viral & Gene Therapy, Shanghai 200438, Peoples R China.,qianqj@sino-gene.cn
英文摘要	Gene-viral therapy, which uses replication-selective transgene-expressing viruses to manage tumors, can exploit the virtues of gene therapy and virotherapy and overcome the limitations of conventional gene therapy. Using a human telomerase reverse transcriptase-targeted replicative adenovirus as an antiangiogenic gene transfer vector to target new angiogenesis and making use of its unrestrained proliferation are completely new concepts in tumor management. CNHK300-mE is a selective replication transgene-expressing adenovirus constructed to carry mouse endostatin gene therapeutically. Infection with CNHK300-mE was associated with selective replication of the adenovirus and production of mouse endostatin in telomerase-positive cancer cells. Endostatin secreted from a human gastric cell line, SGC-7901, infected with CNHK300-mE was significantly higher than that infected with nonreplicative adenovirus Ad-mE in vitro (800 +/- 94.7 ng/ml versus 132.9 +/- 9.9 ng/ml) and in vivo (610 +/- 42 ng/ml versus 126 +/- 13 ng/ml). Embryonic chorioallantoic membrane assay showed that the mouse endostatin secreted by CNHK300-mE inhibited angiogenesis efficiently and also induced distortion of pre-existing vasculature. CNHK300-mE exhibited a superior suppression of xenografts in nude mice compared with CNHK300 and Ad-mE. In summary, we provided a more efficient gene-viral therapy strategy by combining oncolysis with antiangiogenesis.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	TUMOR-GROWTH ; ANTICANCER AGENTS ; BREAST-CANCER ; NUDE-MICE ; BAX GENE ; IN-VIVO ; PROMOTER ; CELLS ; DELIVERY ; ANGIOGENESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000223038200050
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2166]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, Q,Nie, MM,Sham, J,et al. Effective gene-viral therapy for telomerase-positive cancers by selective replicative-competent adenovirus combining with endostatin gene[J]. CANCER RESEARCH,2004,64(15):5390-5397.
APA	Zhang, Q.,Nie, MM.,Sham, J.,Su, CQ.,Xue, HB.,...&Qian, QJ.(2004).Effective gene-viral therapy for telomerase-positive cancers by selective replicative-competent adenovirus combining with endostatin gene.CANCER RESEARCH,64(15),5390-5397.
MLA	Zhang, Q,et al."Effective gene-viral therapy for telomerase-positive cancers by selective replicative-competent adenovirus combining with endostatin gene".CANCER RESEARCH 64.15(2004):5390-5397.