The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease

	The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease
	Gan, JH; Yu, L; Wu, J; Xu, H; Choudhary, JS; Blackstock, WP; Liu, WY; Xia, ZX
刊名	STRUCTURE
	2004
卷号	12 期号:6 页码:1015-1025
通讯作者	Xia, ZX (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China.,xiazx@mail.sioc.ac.cn
英文摘要	Trichomaglin is a protein isolated from root tuber of the plant Maganlin (Trichosanthes Lepiniate, Cucurbitaceae). The crystal structure of trichomaglin has been determined by multiple-isomorphous replacement and refined at 2.2 Angstrom resolution. The X-ray sequence was established, based on electron density combined with the experimentally determined N-terminal sequence, and the sequence information derived from mass spectroscopic analysis. X-ray sequence-based homolog search and the three-dimensional structure reveal that trichomaglin is a novel S-like RNase, which was confirmed by biological assay. Trichomaglin molecule contains an additional beta sheet in the HVb region, compared with the known plant RNase structures. Fourteen cystein residues form seven disulfide bridges, more than those in the other known structures of S- and S-like RNases. His43 and His105 are expected to be the catalytic acid and base, respectively. Four hydrosulfate ions are bound in the active site pocket, three of them mimicking the substrate binding sites.
学科主题	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	GAMETOPHYTIC SELF-INCOMPATIBILITY ; RIBOSOME-INACTIVATING PROTEIN ; GOURD MOMORDICA-CHARANTIA ; AMINO-ACID-SEQUENCE ; CRYSTAL-STRUCTURE ; ANGSTROM RESOLUTION ; NICOTIANA-ALATA ; RHIZOPUS-NIVEUS ; SEEDS
收录类别	SCI
语种	英语
WOS记录号	WOS:000222155100013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2102]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gan, JH,Yu, L,Wu, J,et al. The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease[J]. STRUCTURE,2004,12(6):1015-1025.
APA	Gan, JH.,Yu, L.,Wu, J.,Xu, H.,Choudhary, JS.,...&Xia, ZX.(2004).The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease.STRUCTURE,12(6),1015-1025.
MLA	Gan, JH,et al."The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease".STRUCTURE 12.6(2004):1015-1025.