Multiple signaling pathways control Tbx6 expression during Xenopus myogenesis

	Multiple signaling pathways control Tbx6 expression during Xenopus myogenesis
	Fang, PF; Hu, RY; He, XY; Ding, XY
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2004
卷号	36 期号:6 页码:390-396
关键词	Tbx6 FGF BMP activin/nodal VegT/nodal
通讯作者	Ding, XY (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,xyding@sunm.shcnc.ac.cn
英文摘要	Tbx6 is critical for somite specification and myogenesis initiation. It has been shown that Activin/Nodal, VegT/Nodal, FGF, and BMP signaling pathways are involved early in specifying mesoderm or later in patterning mesoderm, and Xnot plays roles in setting up the boundary between notochord and paraxial mesoderm. In this study, we introduce the dominant negative form of above genes into embryos to evaluate if they are responsible for regulating Tbx6 expression. The results show that: (1) Activin/Nodal and VegT/Nodal signals are necessary for both initiation and maintenance of Tbx6 expression, and Nodal is sufficient to induce ectopic Tbx6 expression; (2) FGF signal is necessary for the initiation and maintenance of Tbx6, but it is not sufficient to induce Tbx6 expression; (3) BMP is also necessary for the expression of Tbx6, and the induction of Tbx6 expression by BMP is dose dependent; (4) Xnot has no effect on the expression of Tbx6. Our results suggest that several signaling pathways are involved in regulating Tbx6 expression, and pave the route to reveal the molecular mechanism of initiating myogenesis.
学科主题	Biochemistry & Molecular Biology ; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	T-BOX GENES ; MESODERM INDUCTION ; EARLY EMBRYOGENESIS ; EMBRYOS ; FGF ; BMP-4 ; GASTRULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000222254900003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2097]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Fang, PF,Hu, RY,He, XY,et al. Multiple signaling pathways control Tbx6 expression during Xenopus myogenesis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(6):390-396.
APA	Fang, PF,Hu, RY,He, XY,&Ding, XY.(2004).Multiple signaling pathways control Tbx6 expression during Xenopus myogenesis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(6),390-396.
MLA	Fang, PF,et al."Multiple signaling pathways control Tbx6 expression during Xenopus myogenesis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.6(2004):390-396.