Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50

	Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50
	Xia, YF; Ye, BQ; Li, YD; Wang, JG; He, XJ; Lin, XF; Yao, XS; Ma, DW; Slungaard, A; Hebbel, RP
刊名	JOURNAL OF IMMUNOLOGY
	2004
卷号	173 期号:6 页码:4207-4217
通讯作者	Geng, JG (reprint author), Univ Minnesota, Sch Med, Vasc Biol Ctr, MMC 480,14-152 Phillips Wangensteen Bldg,420 Dela, Minneapolis, MN 55455 USA.,gengx011@umn.edu
英文摘要	NF-kappaB is a central transcriptional factor and a pleiotropic regulator of many genes involved in immunological responses. During the screening of a plant extract library of traditional Chinese herbal medicines, we found that NF-kappaB activity was potently inhibited by andrographolide (Andro), an abundant component of the plant Andrographis that has been commonly used as a folk remedy for alleviation of inflammatory disorders in Asia for millennia. Mechanistically, it formed a covalent adduct with reduced cysteine (62) of p50, thus blocking the binding of NF-kappaB oligonucleotide to nuclear proteins. Andro suppressed the activation of NF-kappaB in stimulated endothelial cells, which reduced the expression of cell adhesion molecule E-selectin and prevented E-selectin-mediated leukocyte adhesion under flow. It also abrogated the cytokine- and endotoxin-induced peritoneal deposition of neutrophils, attenuated septic shock, and prevented allergic lung inflammation in vivo. Notably, it had no suppressive effect on IkappaBalpha degradation, p50 and p65 nuclear translocation, or cell growth rates. Our results thus reveal a unique pharmacological mechanism of Andro's protective anti-inflammatory actions.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	IN-VITRO ; PLASMA-CELLS ; PANICULATA ; ADHESION ; ROLES ; DNA ; BINDING ; VIVO ; PERITONITIS ; SUPPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000223878000073
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2094]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xia, YF,Ye, BQ,Li, YD,et al. Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50[J]. JOURNAL OF IMMUNOLOGY,2004,173(6):4207-4217.
APA	Xia, YF.,Ye, BQ.,Li, YD.,Wang, JG.,He, XJ.,...&Geng, JG.(2004).Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50.JOURNAL OF IMMUNOLOGY,173(6),4207-4217.
MLA	Xia, YF,et al."Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50".JOURNAL OF IMMUNOLOGY 173.6(2004):4207-4217.