Timing of establishment of paternal methylation imprints in the mouse

	Timing of establishment of paternal methylation imprints in the mouse
	Li, JY; Lees-Murdock, DJ; Xu, GL; Walsh, CP
刊名	GENOMICS
	2004
卷号	84 期号:6 页码:952-960
关键词	germ cells DNA methylation Rasgrf1 Gtl2 differentially methylated region mouse
通讯作者	Walsh, CP (reprint author), Univ Ulster, Sch Biomed Sci, Canc & Ageing Res Grp, Coleraine BT52 1SA, Londonderry, North Ireland.,cp.walsh@ulster.ac.uk
英文摘要	Imprinted genes are characterized by predominant expression from one parental allele and differential DNA methylation. Few imprinted genes have been found to acquire a methylation mark in the male germ line, however, and only one of these, H19, has been studied in detail. We examined methylation of the Rasgif1 and Gtl2 differentially methylated regions (DMR) to determine whether methylation is erased in male germ cells at c12.5 and when the paternal allele acquires methylation. We also compared their methylation dynamics with those of H19 and the maternally methylated gene Snrpn. Our results show that methylation is erased on Rasgrf1, H19, and Snrpn at e12.5, but that Gt12 retains substantial methylation at this stage. Erasure of methylation marks on Gt12 appears to occur later in female germ cells to give the unmethylated profile seen in mature MII oocytes. In the male germ line, de novo methylation of Rasgrf1, Gtl2, and H19 occurs in parallel between e12.5 and e17.5, but the DMR are not completely methylated until the mature sperm stage, suggesting a methylation dynamic different from that of IAP, L1, and minor satellite sequences, which have been shown to become fully methylated by e17.5 in male germ cells. This study also indicates important differences between different imprinted DMR in timing and extent of methylation in the germ cells. (C) 2004 Elsevier Inc. All rights reserved.
学科主题	Biotechnology & Applied Microbiology; Genetics & Heredity
类目[WOS]	Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]	PRIMORDIAL GERM-CELLS ; CYTOSINE METHYLATION ; DNA METHYLATION ; MAMMALIAN DEVELOPMENT ; SNRPN GENE ; LOCUS ; REGION ; MOUSE-CHROMOSOME-12 ; DYNAMICS ; IGF2-H19
收录类别	SCI
语种	英语
WOS记录号	WOS:000225306600006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2093]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, JY,Lees-Murdock, DJ,Xu, GL,et al. Timing of establishment of paternal methylation imprints in the mouse[J]. GENOMICS,2004,84(6):952-960.
APA	Li, JY,Lees-Murdock, DJ,Xu, GL,&Walsh, CP.(2004).Timing of establishment of paternal methylation imprints in the mouse.GENOMICS,84(6),952-960.
MLA	Li, JY,et al."Timing of establishment of paternal methylation imprints in the mouse".GENOMICS 84.6(2004):952-960.