Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma

	Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma
	Song, H; Xia, SL; Liao, C; Li, YL; Wang, YF; Li, TP; Zhao, MJ
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2004
卷号	10 期号:4 页码:509-513
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, POB 35,320 Yue Yang Rd, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn
英文摘要	AIM: To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray. METHODS: The (33)P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue. After a global analysis of gene expression of 8400 genes, we selected some genes to confirm the differential expression using Northern blot and RT-PCR. RESULTS: Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in which about 500 genes were differentially expressed in the paired liver tumor tissues. We identified 4 genes, the expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. In addition, the expression of these genes in 6 hepatoma cell lines was also showed by RT-PCR analysis. CONCLUSION: cDNA microarray permits a high throughput identification of changes in gene expression. The genes encoding Beclin 1, RbAp48, Pir51 and aldolase b are first reported that may be related with hepatocarcinoma.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
收录类别	SCI
语种	英语
WOS记录号	WOS:000208096000009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2084]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Song, H,Xia, SL,Liao, C,et al. Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(4):509-513.
APA	Song, H.,Xia, SL.,Liao, C.,Li, YL.,Wang, YF.,...&Zhao, MJ.(2004).Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,10(4),509-513.
MLA	Song, H,et al."Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 10.4(2004):509-513.