Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein

	Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein
	Liang, Y; Yu, W; Li, Y; Yang, ZY; Yan, XM; Huang, QP; Zhu, XL
刊名	JOURNAL OF CELL BIOLOGY
	2004
卷号	164 期号:4 页码:557-566
关键词	NudE organelle motility time-lapse microscopy RNA interference
通讯作者	Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn
英文摘要	Nudel and Lis1 appear to regulate cytoplasmic dynein in neuronal migration and mitosis through direct interactions. However, whether or not they regulate other functions of dynein remains elusive. Herein, overexpression of a Nudel mutant defective in association with either Lis1 or dynein heavy chain is shown to cause dispersions of membranous organelles whose trafficking depends on dynein. In contrast, the wild-type Nudel and the double mutant that binds to neither protein are much less effective. Time-lapse microscopy for lysosomes reveals significant reduction in both frequencies and velocities of their minus end-directed motions in cells expressing the dynein-binding defective mutant, whereas neither the durations of movement nor the plus end-directed motility is considerably altered. Moreover, silencing Nuclei expression by RNA interference results in Golgi apparatus fragmentation and cell death. Together, it is concluded that Nudel is critical for dynein motor activity in membrane transport and possibly other cellular activities through interactions with both Lis1 and dynein heavy chain.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ASPERGILLUS-NIDULANS ; NEURONAL MIGRATION ; NUCLEAR MIGRATION ; PROTEIN-TRANSPORT ; DYNACTIN COMPLEX ; MITOTIC SPINDLE ; HEAVY-CHAIN ; GOLGI ; CELLS ; MOTOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000189077200022
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2083]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liang, Y,Yu, W,Li, Y,et al. Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein[J]. JOURNAL OF CELL BIOLOGY,2004,164(4):557-566.
APA	Liang, Y.,Yu, W.,Li, Y.,Yang, ZY.,Yan, XM.,...&Zhu, XL.(2004).Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein.JOURNAL OF CELL BIOLOGY,164(4),557-566.
MLA	Liang, Y,et al."Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein".JOURNAL OF CELL BIOLOGY 164.4(2004):557-566.