Identification of glutamate transporters and receptors in mouse testis

	Identification of glutamate transporters and receptors in mouse testis
	Hu, JH; Yang, N; Ma, YH; Jiang, J; Zhang, JF; Fei, J; Guo, LH
刊名	ACTA PHARMACOLOGICA SINICA
	2004
卷号	25 期号:3 页码:366-371
关键词	glutamates receptors amino acid transport systems testis uptake
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,feijian@sunm.shcnc.ac.cn ; mhzhang@sunm.shcnc.ac.cn
英文摘要	AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transporters in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. V(max) value of glutamate uptake was (1.60 +/- 0.21) pmol/min per Ing protein and K(m) value of glutamate uptake was (11.0 +/- 1.6) mumol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR213, GluR6 and KA2) were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.
学科主题	Chemistry; Pharmacology & Pharmacy
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
关键词[WOS]	GAMMA-AMINOBUTYRIC-ACID ; RAT TESTIS ; EXPRESSION ; SPERMATOZOA ; GABA ; DECARBOXYLASE ; EPIDIDYMIS ; SUBUNITS ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000220117800019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2058]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, JH,Yang, N,Ma, YH,et al. Identification of glutamate transporters and receptors in mouse testis[J]. ACTA PHARMACOLOGICA SINICA,2004,25(3):366-371.
APA	Hu, JH.,Yang, N.,Ma, YH.,Jiang, J.,Zhang, JF.,...&Guo, LH.(2004).Identification of glutamate transporters and receptors in mouse testis.ACTA PHARMACOLOGICA SINICA,25(3),366-371.
MLA	Hu, JH,et al."Identification of glutamate transporters and receptors in mouse testis".ACTA PHARMACOLOGICA SINICA 25.3(2004):366-371.