Interaction of polysaccharides with interferon-gamma using an improved ELISA approach

	Interaction of polysaccharides with interferon-gamma using an improved ELISA approach
	Feng, WY; Zhao, LH; Wang, KY
刊名	CARBOHYDRATE POLYMERS
	2004
卷号	58 期号:1 页码:89-94
关键词	heparin heparin-BSA interferon-gamma polysaccharides ELISA
通讯作者	Zhao, LH (reprint author), Zhejiang Univ, Coll Med, Dept Biochem & Mol Biol, 353 Num Yanan Rd, Hangzhou 310031, Peoples R China.,zhaoluhang@263.net
英文摘要	Establishes an ELISA approach to study the interaction of polysaccharides with cytokine in vitro. Synthesizes and separates heparin-BSA complex, after identification of the complex via SDS-PAGE, the ELISA plate wells are coated with HBC, detecting the interaction of interferon-gamma (IFN-gamma) with heparin-BSA complex. We have investigated recombinant human IFN-gamma (rhIFN-gamma) binds to heparin in a concentration-dependent manner. Free heparin and low molecular weight heparin (LMW heparin) competes for the binding of IFN-gamma to heparin, the IC50 concentrations give 2.40 and 18.60 mug/ml, respectively. Other polysaccharides either with sulfate group or without sulfate group compete for the binding of IFN-gamma to HBC with significant different ability. The results suggest that IFN-gamma is a cytokine binding to some polysaccharides including heparin, chondroitin sulfate, fucoidan and carrageenans family with different affinity. ELISA is a simple, sensitive approach to detect the interaction of cytokine with polysaccharides. (C) 2004 Elsevier Ltd. All rights reserved.
学科主题	Chemistry ; Polymer Science
类目[WOS]	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
关键词[WOS]	HUMAN RECOMBINANT INTERLEUKIN-2 ; MOLECULAR-WEIGHT HEPARIN ; SULFATED POLYSACCHARIDES ; BINDING ; CARRAGEENAN ; INHIBITION ; GLYCOSAMINOGLYCANS ; INFLAMMATION ; REPLICATION ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000224145600012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2008]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Feng, WY,Zhao, LH,Wang, KY. Interaction of polysaccharides with interferon-gamma using an improved ELISA approach[J]. CARBOHYDRATE POLYMERS,2004,58(1):89-94.
APA	Feng, WY,Zhao, LH,&Wang, KY.(2004).Interaction of polysaccharides with interferon-gamma using an improved ELISA approach.CARBOHYDRATE POLYMERS,58(1),89-94.
MLA	Feng, WY,et al."Interaction of polysaccharides with interferon-gamma using an improved ELISA approach".CARBOHYDRATE POLYMERS 58.1(2004):89-94.