The human plasma proteome: Analysis of Chinese serum using shotgun strategy

	The human plasma proteome: Analysis of Chinese serum using shotgun strategy
	He, P; He, HZ; Dai, J; Wang, Y; Sheng, QH; Zhou, LP; Zhang, ZS; Sun, YL; Liu, F; Wang, K
刊名	PROTEOMICS
	2005
卷号	5 期号:13 页码:3442-3453
关键词	biomarkers mass spectrometry reference specimen serum proteome shotgun proteomics
通讯作者	Zhao, XH (reprint author), CAMS, Natl Lab Mol Oncol, Canc Inst & Hosp, POB 2258, Beijing, Peoples R China.,zhaoxh@pubem.cicams.ac.cn
英文摘要	We have investigated the serum proteome of Han-nationality Chinese by using shotgun strategy. A complete proteomics analysis was performed on two reference specimens from a total of 20 healthy donors, in which each sample was made from ten-pooled male or female serum, respectively. The methodology used encompassed (1) removal of six high-abundant proteins; (2) tryptic digestion of low- and high-abundant proteins of serum; (3) separation of peptide mixture by RP-HPLC followed by ESI-MS/MS identification. A total of 944 nonredundant proteins were identified under a stringent filter condition (X-corr >= 1.9, >= 2.2, and >= 3.75, Delta Cn >= 0.1, and R-sp <= 4.0) in both pooled male and female samples, in which 594 and 622 entire proteins were found, respectively. Compared with the total 3020 protein identifications confirmed by more than one laboratory or more than one specimen in HUPO Plasma Proteome Project (PPP) participating laboratories recently, 206 proteins were identified with at least two distinct peptides per protein and 185 proteins were considered as high-confidence identification. Moreover, some lower abundance serum proteins (ng/mL range) were detected, such as complement C5 and CA125, routinely used as an ovarian cancer marker in plasma and serum. The resulting nonredundant list of serum proteins would add significant information to the knowledge base of human plasma proteome and facilitate disease markers discovery.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
关键词[WOS]	2-DIMENSIONAL ELECTROPHORESIS ; RAT SERUM ; MASS-SPECTROMETRY ; IDENTIFICATION TECHNOLOGY ; PROTEINS ; PHASE ; MODULATION ; EXPRESSION ; MAP
收录类别	SCI
语种	英语
WOS记录号	WOS:000231477600019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1969]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	He, P,He, HZ,Dai, J,et al. The human plasma proteome: Analysis of Chinese serum using shotgun strategy[J]. PROTEOMICS,2005,5(13):3442-3453.
APA	He, P.,He, HZ.,Dai, J.,Wang, Y.,Sheng, QH.,...&Zhao, XH.(2005).The human plasma proteome: Analysis of Chinese serum using shotgun strategy.PROTEOMICS,5(13),3442-3453.
MLA	He, P,et al."The human plasma proteome: Analysis of Chinese serum using shotgun strategy".PROTEOMICS 5.13(2005):3442-3453.