Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death

	Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death
	Yang, ZY; Guo, J; Chen, Q; Ding, C; Du, J; Zhu, XL
刊名	MOLECULAR AND CELLULAR BIOLOGY
	2005
卷号	25 期号:10 页码:4062-4074
通讯作者	Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Lab Mol Cell Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn
英文摘要	Mitosin (also named CENP-F) is a large human nuclear protein transiently associated with the outer kinetochore plate in M phase. Using RNA interference and fluorescence microscopy, we showed that mitosin depletion attenuated chromosome congression and led to metaphase arrest with misaligned polar chromosomes whose kinetochores showed few cold-stable microtubules. Kinetochores of fully aligned chromosomes often failed to show orientation in the direction of the spindle long axis. Moreover, tension across their sister kinetochores was decreased by 53% on average. These phenotypes collectively imply defects in motor functions in mitosin-depleted cells and are similar to those of CENP-E depletion. Consistently, the intensities of CENP-E and cytoplasmic dynein and dynactin, which are motors controlling microtubule attachment and chromosome movement, were reduced at the kinetochore in a microtubule-dependent manner. In addition, after being arrested in pseudometaphase for approximately 2 h, mitosin-depleted cells died before anaphase initiation through apoptosis. The dying cells exhibited progressive chromosome arm decondensation, while the centromeres were still associated with spindles. Mitosin is therefore essential for full chromosome alignment, possibly by promoting proper kinetochore attachments through modulating CENP-E and dynein functions. Its depletion also prematurely triggers chromosome decondensation, a process that normally occurs from telophase for the nucleus reassembly, thus resulting in apoptosis.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	KINETOCHORE-MICROTUBULE ATTACHMENT ; CENP-E ; CYTOPLASMIC DYNEIN ; SPINDLE CHECKPOINT ; MAMMALIAN-CELLS ; PROTEIN-TRANSPORT ; LIVING CELLS ; HELA-CELLS ; LOCALIZATION ; DYNAMICS
收录类别	SCI
语种	英语
WOS记录号	WOS:000228888100019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1960]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, ZY,Guo, J,Chen, Q,et al. Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death[J]. MOLECULAR AND CELLULAR BIOLOGY,2005,25(10):4062-4074.
APA	Yang, ZY,Guo, J,Chen, Q,Ding, C,Du, J,&Zhu, XL.(2005).Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death.MOLECULAR AND CELLULAR BIOLOGY,25(10),4062-4074.
MLA	Yang, ZY,et al."Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death".MOLECULAR AND CELLULAR BIOLOGY 25.10(2005):4062-4074.