Transient overexpression of gamma interferon promotes Aspergillus clearance in invasive pulmonary aspergillosis

	Transient overexpression of gamma interferon promotes Aspergillus clearance in invasive pulmonary aspergillosis
	Shao, C; Qu, J; He, L; Zhang, Y; Wang, J; Wang, Y; Zhou, H; Liu, X
刊名	CLINICAL AND EXPERIMENTAL IMMUNOLOGY
	2005
卷号	142 期号:2 页码:233-241
关键词	fungi invasive pulmonary aspergillosis gene therapy interferon gamma adenovirus
通讯作者	Qu, J (reprint author), Fudan Univ, Zhongshan Hosp, Dept Pulm Med, Dept Resp Med, 180 Feng Lin Rd, Shanghai 200032, Peoples R China.,jiemingqu@126.com
英文摘要	Cytokines are critical molecules necessary for normal lung pathogen host defences. Gamma interferon (IFN-gamma) and T1-phenotype immune responses are important components of host defence against Aspergillus. Therefore, we hypothesized that transient overexpression of IFN-gamma within the lung could augment host immunity against Aspergillus. Here it was showed that intranasal administration of 5 x 10(7) colony-forming units (CFU) of Aspergillus fumigatus (Af ) induced the expression of IFN-gamma. Mice were intranasally (i.n) administrated with 5 x 10(8) PFU of a recombinant adenovirus vector containing the murine IFN-gamma cDNA (AdmIFN-gamma), and challenged 24 h later with Af. We observed that i.n. administration of AdmIFN-gamma resulted in about a fourfold increase in levels of IFN-gamma and IL-12 within the lung, about a 75% reduction in lung fungal contents at day 2 and a more than threefold higher survival rate in the AdmIFN-gamma-treated group compared to the controls (P < 0.01). This protection effect was not found when AdmIFN-gamma was i.p. administrated. Alveolar macrophages and lung leucocytes isolated from i.n. AdmIFN-gamma-treated animals displayed enhanced killing of intracellular Aspergillus organisms ex vivo. These results demonstrate that transient overexpression of IFN-gamma could augment host defence against Aspergillus.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	HOST-DEFENSE ; GENE-THERAPY ; INNATE IMMUNITY ; TRANSGENIC EXPRESSION ; CHEMOKINE KC ; IFN-GAMMA ; T-CELLS ; MICE ; LUNG ; INFECTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000232657300004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1853]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Shao, C,Qu, J,He, L,et al. Transient overexpression of gamma interferon promotes Aspergillus clearance in invasive pulmonary aspergillosis[J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY,2005,142(2):233-241.
APA	Shao, C.,Qu, J.,He, L.,Zhang, Y.,Wang, J.,...&Liu, X.(2005).Transient overexpression of gamma interferon promotes Aspergillus clearance in invasive pulmonary aspergillosis.CLINICAL AND EXPERIMENTAL IMMUNOLOGY,142(2),233-241.
MLA	Shao, C,et al."Transient overexpression of gamma interferon promotes Aspergillus clearance in invasive pulmonary aspergillosis".CLINICAL AND EXPERIMENTAL IMMUNOLOGY 142.2(2005):233-241.