Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene

	Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene
	Da, L; Li, D; Yokoyama, KK; Tsaiping, L; Zhao, MJ
刊名	BIOCHEMICAL JOURNAL
	2006
卷号	393 期号:1 页码:779-788
关键词	cell death-inducing DFF45 (DNA fragmentation factor 45)-like effector (CIDE) cell-specific expression DNA methylation promoter hepatocyte nuclear factor-4 (HNF4) Sp1
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn
英文摘要	CIDE-B [cell death-inducing DFF45 (DNA fragmentation factor 45)-like effector B] is a member of the CIDE family of apoptosisinducing factors. The highly restricted pattern of expression of CIDE-B in the liver and spleen suggests that a mechanism exists for the tissue- and cell-specific regulation of transcription of this gene. We have analysed the promoters of the human CIDE-B gene, particularly the mechanism of cell-specific transcription. Expression of CIDE-B is driven by two promoters which are responsible for the synthesis of two types of transcript, and Sp1 and Sp3 are key regulators of basal transcription from both the upstream and the internal promoter, as indicated by EMSAs (electrophoretic mobility-shift assays) and site-directed mutagenesis. Bisulphite sequencing analysis demonstrated that the upstream promoter was hypermethylated in cells that did not express the long transcript of CIDE-B, but was hypomethylated in cells that expressed this transcript. Furthermore, methylation of this region in vitro reduced the promoter activity to similar to 5% of the control. Thus methylation at CpG sites in the upstream promoter region appeared to be important for cell-specific synthesis of the long transcript. By contrast, HNF4 alpha (hepatocyte nuclear factor4 alpha) bound to the internal promoter and enhanced its activity. Moreover, the short transcript of CIDE-B gene was expressed in cells which do not normally express this transcript upon introduction of exogenous HNF4 alpha, demonstrating the involvement of HNF4 alpha in the cell-specific synthesis of the short transcript. Thus our analysis revealed a novel mechanism for the cellspecific transcription of the human CIDE-B gene, which involves epigenetic and genetic control at separate respective promoters.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	DNA-FRAGMENTATION-FACTOR ; ADIPOCYTE-SPECIFIC GENE ; WILD-TYPE P53 ; LEUKOTRIENE B-4 ; CIDE-B ; TRANSCRIPTIONAL REGULATION ; BINDING-PROTEIN ; APOPTOSIS ; METHYLATION ; RECEPTOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000234947800019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1834]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Da, L,Li, D,Yokoyama, KK,et al. Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene[J]. BIOCHEMICAL JOURNAL,2006,393(1):779-788.
APA	Da, L,Li, D,Yokoyama, KK,Tsaiping, L,&Zhao, MJ.(2006).Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene.BIOCHEMICAL JOURNAL,393(1),779-788.
MLA	Da, L,et al."Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene".BIOCHEMICAL JOURNAL 393.1(2006):779-788.