Structural insights into the specific binding of huntingtin proline-rich region with the SH3 and WW domains

	Structural insights into the specific binding of huntingtin proline-rich region with the SH3 and WW domains
	Gao, YG; Yan, XZ; Song, AX; Chang, YG; Gao, XC; Jiang, N; Zhang, Q; Hu, HY
刊名	STRUCTURE
	2006
卷号	14 期号:12 页码:1755-1765
通讯作者	Yan, XZ (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,yanxz@proteomics.cn
英文摘要	The interactions of huntingtin (Htt) with the SH3 domain-or WW domain-containing proteins have been implicated in the pathogenesis of Huntington's disease (HD). We report the specific interactions of Htt proline-rich region (PRR) with the SH3GL3-SH3 domain and HYPA-WW1-2 domain pair by NMR. The results show that Htt PRR binds with the SH3 domain through nearly its entire chain, and that the binding region on the domain includes the canonical PxxP-binding site and the specificity pocket. The C terminus of PRR orients to the specificity pocket, whereas the N terminus orients to the PxxP-binding site. Htt PRR can also specifically bind to WW1-2; the N-terminal portion preferentially binds to WW1, while the C-terminal portion binds to WW2. This study provides structural insights into the specific interactions between Htt PRR and its binding partners as well as the alteration of these interactions that involve PRR, which may have implications for the understanding of HD.
学科主题	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	DISEASE PATHOGENESIS ; NMR RELAXATION ; PROTEIN ; RECOGNITION ; LIGANDS ; INTERACTS ; SYSTEM ; AGGREGATION ; RESTRAINTS ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000242888600004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1784]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gao, YG,Yan, XZ,Song, AX,et al. Structural insights into the specific binding of huntingtin proline-rich region with the SH3 and WW domains[J]. STRUCTURE,2006,14(12):1755-1765.
APA	Gao, YG.,Yan, XZ.,Song, AX.,Chang, YG.,Gao, XC.,...&Hu, HY.(2006).Structural insights into the specific binding of huntingtin proline-rich region with the SH3 and WW domains.STRUCTURE,14(12),1755-1765.
MLA	Gao, YG,et al."Structural insights into the specific binding of huntingtin proline-rich region with the SH3 and WW domains".STRUCTURE 14.12(2006):1755-1765.