The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron

	The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron
	Wang, LC; Xiong, W; Zheng, J; Zhou, Y; Zheng, H; Zhang, C; Zheng, LH; Zhu, XL; Xiong, ZQ; Wang, LY
刊名	BIOPHYSICAL JOURNAL
	2006
卷号	90 期号:10 页码:3590-3598
通讯作者	Zhou, Z (reprint author), Peking Univ, Inst Mol Med, 5 Yiheyuan Rd, Beijing 100871, Peoples R China.,zzhou@pku.edu.cn
英文摘要	Endocytosis is a fundamental cellular event in membrane retrieval after exocytosis and in the regulation of receptor-mediated signal transduction. In contrast to the well-studied depolarization-induced membrane recycling, little is known about the kinetics of ligand-induced endocytosis of G-protein-coupled receptors in neurons. Here we investigated the kinetics of ligand-receptor binding-induced endocytosis in rat sensory neurons using a membrane capacitance assay. The time constant of ADP-induced endocytosis of P2Y-receptors was determined as 1.7 s. The ADP-induced endocytosis was blocked by antagonists against P2Y, phosphorylation, and clathrin. However, block of dynamin was without effect. The ADP-induced endocytosis was confirmed independently by a single vesicle image technique using a styryl FM2-10. Finally, the receptors were internalized in response to ADP, as determined by GFP-labeled P2Y. We conclude that ligand-receptor binding leads to rapid endocytosis in the cytoplasm of rat dorsal root ganglion neurons.
学科主题	Biophysics
类目[WOS]	Biophysics
关键词[WOS]	ROOT GANGLION NEURONS ; ADRENAL CHROMAFFIN CELLS ; CLATHRIN-MEDIATED ENDOCYTOSIS ; FROG NEUROMUSCULAR JUNCTION ; TRANSMITTER RELEASE ; RAPID ENDOCYTOSIS ; NERVE-TERMINALS ; CENTRAL SYNAPSE ; CALCIUM INFLUX ; RAT ADIPOCYTES
收录类别	SCI
语种	英语
WOS记录号	WOS:000236901400021
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1768]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, LC,Xiong, W,Zheng, J,et al. The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron[J]. BIOPHYSICAL JOURNAL,2006,90(10):3590-3598.
APA	Wang, LC.,Xiong, W.,Zheng, J.,Zhou, Y.,Zheng, H.,...&Zhou, Z.(2006).The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron.BIOPHYSICAL JOURNAL,90(10),3590-3598.
MLA	Wang, LC,et al."The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron".BIOPHYSICAL JOURNAL 90.10(2006):3590-3598.