Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp)

	Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp)
	Jin, XL; Tao, ZJ; Jia, J; He, XX; Jin, YX
刊名	CHINESE SCIENCE BULLETIN
	2006
卷号	51 期号:7 页码:824-829
关键词	species-specific aminoacylation tRNA(Trp) tryptophanyl-tRNA synthetase (TrpRS) O. sativa mitochondrial
通讯作者	He, XX (reprint author), Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China.,jhhxx@zjnu.cn ; yxjin@sibs.ac.cn
英文摘要	The details of species-specific aminoacylation in Oryza sativa mitochondrial tRNA(TrP) by bacterial and eukaryotic (cytoplasm) tryptophanyl-tRNA synthetases (TrpRS) were investigated. Seven single or multiple mutations of three bases (G73, U72, A 68) were made in O.sativa mitochondrial tRNA(Trp) to the corresponding nucleotides present in human tRNA(Trp). In vitro transcripts of these mutant genes were tryptophanylated by Bacillus subtilis and human tryptophanyl-tRNA synthetases (TrpRS), and the kinetic parameters were determined. The results showed that the aminoacylation of seven mutant transcripts by B. subtilis TrpRS was 53.33%-99.79% less efficient than that by wild-type O.sativa mitochondrial tRNA(Trp), but was 4-330 times more efficient than that by human TrpRS. The mutant MPH7 (G73, U72 and C68 in O.sativa mitochondrial tRNA were all replaced by the counterpart residues from human tRNA(Trp) and showed a great change in aminoacylation efficiency. Our results inclicate that the species-specific identity elements of O.sativa mitochondrial tRNA(Trp) are similar to bacterial and eukaryotic (cytoplasm). They are mainly located at the discriminator base, the first and the fifth pairs of bases, the discriminator base G73, two bases in the acceptor stem G1/U72 and U5/A68. Our results also provide new data in support of the hypothesis that mitochondrial tRNA Trp is of eubacterial origin.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	TRANSFER-RNA-SYNTHETASE ; BACILLUS-SUBTILIS ; IDENTITY ; RECOGNITION ; RULES ; CODE
收录类别	SCI
语种	英语
WOS记录号	WOS:000237493500010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1743]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jin, XL,Tao, ZJ,Jia, J,et al. Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp)[J]. CHINESE SCIENCE BULLETIN,2006,51(7):824-829.
APA	Jin, XL,Tao, ZJ,Jia, J,He, XX,&Jin, YX.(2006).Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp).CHINESE SCIENCE BULLETIN,51(7),824-829.
MLA	Jin, XL,et al."Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp)".CHINESE SCIENCE BULLETIN 51.7(2006):824-829.