Second intron of mouse nestin gene directs its expression in pluripotent embryonic carcinoma cells through POU factor binding site

	Second intron of mouse nestin gene directs its expression in pluripotent embryonic carcinoma cells through POU factor binding site
	Jin, ZG; Liu, L; Zhong, H; Zhang, KJ; Chen, YF; Bian, W; Cheng, LP; Jing, NH
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2006
卷号	38 期号:3 页码:207-212
关键词	nestin embryonic carcinoma cells enhancer POU factor
通讯作者	Jing, NH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Stem Cell Biol,Lab Mol Cell Biol, Shanghai 200031, Peoples R China.,njing@sibs.ac.cn
英文摘要	Nestin, an intermediate filament protein, is expressed in the neural stem cells of the developing central nervous system. This tissue-specific expression is driven by the neural stem cell-specific enhancer in the second intron of the nestin gene. In this study, we showed that the mouse nestin gene was expressed in pluripotent embryonic carcinoma (EC) P19 and F9 cells, not in the differentiated cell types. This cell type-specific expression was conferred by the enhancer in the second intron. Mutation of the conserved POU factor-binding site in the enhancer abolished the reporter gene expression in EC cells. Oct4, a Class V POU factor, was found to be coexpressed with nestin in EC cells. Electrophoretic mobility-shift assays and supershift assays showed that a unique protein-DNA complex was formed specifically with nuclear extracts of EC cells, and Oct4 protein was included. Together, these results suggest the functional relevance between the conserved POU factor-binding site and the expression of the nestin gene in pluripotent EC cells.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	NEURAL STEM-CELLS ; TRANSCRIPTION FACTOR ; PROGENITOR CELLS ; TRANSGENIC MICE ; SELF-RENEWAL ; ES CELLS ; PROTEIN ; DIFFERENTIATION ; LINEAGES ; PROMOTER
收录类别	SCI
语种	英语
WOS记录号	WOS:000235937800009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1686]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jin, ZG,Liu, L,Zhong, H,et al. Second intron of mouse nestin gene directs its expression in pluripotent embryonic carcinoma cells through POU factor binding site[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(3):207-212.
APA	Jin, ZG.,Liu, L.,Zhong, H.,Zhang, KJ.,Chen, YF.,...&Jing, NH.(2006).Second intron of mouse nestin gene directs its expression in pluripotent embryonic carcinoma cells through POU factor binding site.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(3),207-212.
MLA	Jin, ZG,et al."Second intron of mouse nestin gene directs its expression in pluripotent embryonic carcinoma cells through POU factor binding site".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.3(2006):207-212.