A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate

	A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate
	Hong-Tao, W; Shao-Ling, Z; Wang-Yi, L
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2007
卷号	34 期号:9 页码:991-995
关键词	ribosome-inactivating protein(RIP) double-stranded supercoiled DNA gel electrophoresis
通讯作者	Shao-Ling, Z (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,nnzsl@njau.edu.cn ; wyliu@sibs.ac.an
英文摘要	A novel method for assaying the enzymatic activity of ribosome-inactivating proteins (RIPs) has been developed. The principle of the method is based on that RIP can remove some adenine bases from double-stranded supercoiled DNA molecules, subsequently, the deadenylated DNA was cleaved into nicked and linear form. After treatment with acidic aniline, the deadenylated DNA was degraded into many small fragments, and run out of the gel. The enzymatic activities of two RIPs (trichosanthin and cinnamomin) were tested using this method, the limit of sensitivity is about 50 ng (trichosanthin) and 5 ng (reduced cinnamomin). It should be emphasized that the merit of this method is to avoid the preparation of ribosome.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	RNA N-GLYCOSIDASE ; EUKARYOTIC RIBOSOMES ; CINNAMOMUM-CAMPHORA ; SUPERCOILED DNA ; MECHANISM ; RICIN ; CHAIN
收录类别	SCI
语种	中文
WOS记录号	WOS:000249601800013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1596]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hong-Tao, W,Shao-Ling, Z,Wang-Yi, L. A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(9):991-995.
APA	Hong-Tao, W,Shao-Ling, Z,&Wang-Yi, L.(2007).A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34(9),991-995.
MLA	Hong-Tao, W,et al."A novel method for assaying enzymatic activity of ribosome-inactivating protein using plasmid DNA as substrate".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34.9(2007):991-995.