Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site

	Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site
	Deng, Q; Zhai, JW; Michel, ML; Zhang, J; Qin, J; Kong, YY; Zhang, XX; Budkowska, A; Tiollais, P; Wang, Y
刊名	JOURNAL OF VIROLOGY
	2007
卷号	81 期号:8 页码:4244-4254
通讯作者	Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,wangy@sibs.ac.cn ; yhxie@sibs.ac.cn
英文摘要	A direct involvement of the PreS domain of the hepatitis B virus (HBV) large envelope protein, and in particular amino acid residues 21 to 47, in virus attachment to hepatocytes has been suggested by many previous studies. Several PreS-interacting proteins have been identified. However, they share few common sequence motifs, and a bona fide cellular receptor for HBV remains elusive. In this study, we aimed to identify PreS-interacting motifs and to search for novel HBV-interacting proteins and the long-sought receptor. PreS fusion proteins were used as baits to screen a phage display library of random peptides. A group of PreS-binding peptides were obtained. These peptides could bind to amino acids 21 to 47 of PreS1 and shared a linear motif (W(1)T(2)X(3)W(4)W(5)) sufficient for binding specifically to PreS and viral particles. Several human proteins with such a motif were identified through BLAST search. Analysis of their biochemical and structural properties suggested that lipoprotein lipase (LPL), a key enzyme in lipoprotein metabolism, might interact with PreS and HBV particles. The interaction of HBV with LPL was demonstrated by in vitro binding, virus capture, and cell attachment assays. These findings suggest that LPL may play a role in the initiation of HBV infection. Identification of peptides and protein ligands corresponding to LPL that bind to the HBV envelope will offer new therapeutic strategies against HBV infection.
学科主题	Virology
类目[WOS]	Virology
关键词[WOS]	LIPOPROTEIN-LIPASE GENE ; X-RAY-STRUCTURE ; PHAGE DISPLAY ; TRANSMEMBRANE TOPOLOGY ; CHYLOMICRON REMNANTS ; RICH LIPOPROTEINS ; APOLIPOPROTEIN-H ; HUMAN PLASMA ; THP-1 CELLS ; C VIRUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000245692900055
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1593]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Deng, Q,Zhai, JW,Michel, ML,et al. Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site[J]. JOURNAL OF VIROLOGY,2007,81(8):4244-4254.
APA	Deng, Q.,Zhai, JW.,Michel, ML.,Zhang, J.,Qin, J.,...&Xie, YH.(2007).Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site.JOURNAL OF VIROLOGY,81(8),4244-4254.
MLA	Deng, Q,et al."Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site".JOURNAL OF VIROLOGY 81.8(2007):4244-4254.