Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment

	Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment
	Li, Y; Yu, W; Liang, Y; Zhu, XL
刊名	CELL RESEARCH
	2007
卷号	17 期号:8 页码:701-712
关键词	kinetochore cytoplasmic dynein chromosome mitosis force
通讯作者	Zhu, XL (reprint author), Chinese Acad Sci, Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn
英文摘要	For proper chromosome segregation, all kinetochores must achieve bipolar microtubule (MT) attachment and subsequently align at the spindle equator before anaphase onset. The MT minus end-directed motor dynein/dynactin binds kinetochores in prometaphase and has long been implicated in chromosome congression. Unfortunately, inactivation of dynein usually disturbs spindle organization, thus hampering evaluation of its kinetochore roles. Here we specifically eliminated kinetochore dynein/dynactin by RNAi-mediated depletion of ZW 10, a protein essential for kinetochore localization of the motor. Time-lapse microscopy indicated markedly-reduced congression efficiency, though congressing chromosomes displayed similar velocities as in control cells. Moreover, cells frequently failed to achieve full chromosome alignment, despite their normal spindles. Confocal microcopy revealed that the misaligned kinetochores were monooriented or unattached and mostly lying outside the spindle, suggesting a difficulty to capture MTs from the opposite pole. Kinetochores on monoastral spindles were dispersed farther away from the pole and exhibited only mild oscillation. Furthermore, inactivating dynein by other means generated similar phenotypes. Therefore, kinetochore dynein produces on monooriented kinetochores a poleward pulling force, which may contribute to efficient bipolar attachment by facilitating their proper microtubule captures to promote congression as well as full chromosome alignment.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	SMALL-MOLECULE INHIBITOR ; CYTOPLASMIC DYNEIN ; MITOTIC CHECKPOINT ; SPINDLE CHECKPOINT ; MAMMALIAN-CELLS ; CENP-E ; PROTEIN-TRANSPORT ; MITOSIS ; ZW10 ; DYNACTIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000249774500006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1588]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, Y,Yu, W,Liang, Y,et al. Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment[J]. CELL RESEARCH,2007,17(8):701-712.
APA	Li, Y,Yu, W,Liang, Y,&Zhu, XL.(2007).Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment.CELL RESEARCH,17(8),701-712.
MLA	Li, Y,et al."Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment".CELL RESEARCH 17.8(2007):701-712.