Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli

	Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli
	Liang, Y; Yu, W; Li, Y; Yu, LH; Zhang, QG; Wang, FB; Yang, ZY; Du, J; Huang, QP; Yao, XB
刊名	MOLECULAR BIOLOGY OF THE CELL
	2007
卷号	18 期号:7 页码:2656-2666
通讯作者	Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn
英文摘要	The microtubule-based motor cytoplasmic dynein/dynactin is a force generator at the kinetochore. It also transports proteins away from kinetochores to spindle poles. Regulation of such diverse functions, however, is poorly understood. We have previously shown that Nudel is critical for dynein-mediated protein transport, whereas mitosin, a kinetochore protein that binds Nudel, is involved in retention of kinetochore dynein/dynactin against microtubule-dependent stripping. Here we demonstrate that Nudel is required for robust localization of dynein/dynactin at the kinetochore. It localizes to kinetochores after nuclear envelope breakdown, depending mostly (similar to 78%) on mitosin and slightly on dynein/dynactin. Depletion of Nudel by RNA interference (RNAi) or overexpression of its mutant incapable of binding either Lisl or dynein heavy chain abolishes the kinetochore protein transport and mitotic progression. Similar to mitosin RNAi, Nudel RNAi also leads to increased stripping of kinetochore dynein/dynactin in the presence of microtubules. Taking together, our results suggest a dual role of kinetochore Nudel: it activates dynein-mediated protein transport and, when interacting with both mitosin and dynein, stabilizes kinetochore dynein/dynactin against microtubule-dependent stripping to facilitate the force generation function of the motor.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	SPINDLE CHECKPOINT ; MITOTIC SPINDLE ; CENP-F ; MITOSIN/CENP-F ; CHROMOSOME ALIGNMENT ; NEURONAL MIGRATION ; PROTEIN-TRANSPORT ; MAMMALIAN-CELLS ; DYNACTIN ; LIS1
收录类别	SCI
语种	英语
WOS记录号	WOS:000247604000026
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1582]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liang, Y,Yu, W,Li, Y,et al. Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli[J]. MOLECULAR BIOLOGY OF THE CELL,2007,18(7):2656-2666.
APA	Liang, Y.,Yu, W.,Li, Y.,Yu, LH.,Zhang, QG.,...&Zhu, XL.(2007).Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli.MOLECULAR BIOLOGY OF THE CELL,18(7),2656-2666.
MLA	Liang, Y,et al."Nudel modulates kinetochore association and function of cytoplasmic dynein in M phaseli".MOLECULAR BIOLOGY OF THE CELL 18.7(2007):2656-2666.