Overexpression of the gene for transmembrane 4 superfamily member 4 accelerates liver damage in rats treated with CCl4

	Overexpression of the gene for transmembrane 4 superfamily member 4 accelerates liver damage in rats treated with CCl4
	Qiu, J; Liu, ZW; Da, L; Li, Y; Xuan, HX; Lin, QS; Li, F; Wang, YF; Li, ZP; Zhao, MJ
刊名	JOURNAL OF HEPATOLOGY
	2007
卷号	46 期号:2 页码:266-275
关键词	TM4SF4 carbon tetrachloride acute liver injury
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn
英文摘要	Background/Aims: Transmembrane 4 superfamily member 4 (TM4SF4) is up-regulated in regenerating liver after partial hepatectomy in rats, but the in vivo functions of this protein are still largely unknown. Therefore, we investigated the role of TM4SF4 during liver injury. Methods: Expression of TM4SF4 was analyzed by RT-PCR and Western blotting in normal and CCl4-injured rats. Overexpression or reduced expression of TM4SF4 in the liver was achieved by injection of sense or antisense TM4SF4 expression plasmids. Assessment of liver injury (histology, serum ALT and AST levels), apoptosis by TUNEL assay were performed. Expression of injury-related genes was analyzed by quantitative real-time PCR. Results: Overexpression of TM4SF4 in rats after CCl4 treatment showed extensive liver damage and increased levels of serum ALT and AST. Decreased TM4SF4 gene expression showed minimal liver necrosis and depressed ALT and AST levels. Increased expression of TM4SF4 affected the expression levels of growth factors and receptors, such as TNF-alpha, TNFR1 and c-met. Furthermore, pro-apoptotic and anti-apoptotic gene expression was altered after TM4SF4 administration. Conclusions: Rat TM4SF4 is overexpressed in acutely injured liver induced by CCl4 and plays a crucial role in accelerating liver injury, which may be mediated by the TNF-a and HGF/c-met signaling pathways. (c) 2006 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
关键词[WOS]	HEPATIC STELLATE CELLS ; NECROSIS-FACTOR-ALPHA ; GROWTH-FACTOR-BETA ; CARBON-TETRACHLORIDE ; TETRASPANIN PROTEINS ; SIGNALING COMPLEXES ; INJURY ; APOPTOSIS ; EXPRESSION ; FIBROSIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000243813100012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1528]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Qiu, J,Liu, ZW,Da, L,et al. Overexpression of the gene for transmembrane 4 superfamily member 4 accelerates liver damage in rats treated with CCl4[J]. JOURNAL OF HEPATOLOGY,2007,46(2):266-275.
APA	Qiu, J.,Liu, ZW.,Da, L.,Li, Y.,Xuan, HX.,...&Zhao, MJ.(2007).Overexpression of the gene for transmembrane 4 superfamily member 4 accelerates liver damage in rats treated with CCl4.JOURNAL OF HEPATOLOGY,46(2),266-275.
MLA	Qiu, J,et al."Overexpression of the gene for transmembrane 4 superfamily member 4 accelerates liver damage in rats treated with CCl4".JOURNAL OF HEPATOLOGY 46.2(2007):266-275.