UXT is a novel and essential cofactor in the NF-kappa B transcriptional enhanceosome

	UXT is a novel and essential cofactor in the NF-kappa B transcriptional enhanceosome
	Sui, SG; Tang, YJ; Lou, XW; Zhu, LH; Yang, K; Zhang, BH; Shi, HX; Wang, C
刊名	JOURNAL OF CELL BIOLOGY
	2007
卷号	178 期号:2 页码:231-244
通讯作者	Wang, C (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,cwang01@sibs.ac.cn
英文摘要	A s a latent transcription factor, nuclear factor KB (NF-KB) translocates from the cytoplasm into the nucleus upon stimulation and mediates the expression of genes that are important in immunity, inflammation, and development. However, little is known about how it is regulated inside the nucleus. By a two-hybrid approach, we identify a prefoldin-like protein, ubiquitously expressed transcript (UXT), that is expressed predominantly and interacts specifically with NF-kappa B inside the nucleus. RNA interference knockdown of UXT leads to impaired NF-kappa B activity and dramatically attenuates the expression of NF-kappa B-dependent genes. This interference also sensitizes cells to apoptosis by tumor necrosis factor-alpha. Furthermore, UXT forms a dynamic complex with NF-kappa B and is recruited to the NF-kappa B enkanceosome upon stimulation. Interestingly, the UXT protein level correlates with constitutive NF-kappa B activity in human prostate cancer cell lines. The presence of NF-kappa B within the nucleus of stimulated or constitutively active cells is considerably diminished with decreased endogenous UXT levels. Our results reveal that UXT is an integral component of the NF-kappa B enhanceosome and is essential for its nuclear function, which uncovers a new mechanism of NF-kappa B regulation.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ALPHA-INDUCED APOPTOSIS ; PROSTATE-CANCER ; IKK-ALPHA ; CONSTITUTIVE ACTIVATION ; UNFOLDED PROTEINS ; GENE-EXPRESSION ; UBIQUITINATION ; ACETYLATION ; CHAPERONES ; PREFOLDIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000248151500006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1522]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Sui, SG,Tang, YJ,Lou, XW,et al. UXT is a novel and essential cofactor in the NF-kappa B transcriptional enhanceosome[J]. JOURNAL OF CELL BIOLOGY,2007,178(2):231-244.
APA	Sui, SG.,Tang, YJ.,Lou, XW.,Zhu, LH.,Yang, K.,...&Wang, C.(2007).UXT is a novel and essential cofactor in the NF-kappa B transcriptional enhanceosome.JOURNAL OF CELL BIOLOGY,178(2),231-244.
MLA	Sui, SG,et al."UXT is a novel and essential cofactor in the NF-kappa B transcriptional enhanceosome".JOURNAL OF CELL BIOLOGY 178.2(2007):231-244.