CD19(+)CD5(+) B Cells in Primary IgA Nephropathy

	CD19(+)CD5(+) B Cells in Primary IgA Nephropathy
	He, YL; Xiao, RJ; Ji, X; Jiang, YP; Chen, L; Li, L; Yang, DP; Tan, XT; Liu, JY; Tang, ZQ
刊名	JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
	2008
卷号	19 期号:11 页码:2130-2139
通讯作者	Tan, JQ (reprint author), Wuhan Univ, Sch Med, Dept Immunol, Dong Hu Rd 115, Wuhan 430071, Peoples R China.,DGX1010@yahoo.com ; jinquan_tan@whu.edu.cn
英文摘要	The source of IgA and the mechanism for deposition of IgA in the mesangium remain unknown for primary IgA nephropathy. Because CD19(+)CD5(+) B cells are important producers of IgA and contribute to several autoimmune diseases, they may play an important role in IgA nephropathy. In this study, flow cytometry, quantitative PCR, and confocal microscopy were used to assess the frequency, distribution, Ig production, CD phenotypes, cytokine production, and sensitivity to apoptosis of CD19(+)CD5(+) B cells in the peripheral blood, peritoneal fluid, and kidney biopsies of 36 patients with primary IgA nephropathy. All patients with IgA nephropathy were significantly more likely to have CD19(+)CD5(+) B cells in the peripheral blood, peritoneal fluid, and kidney biopsies than were five control subjects and 10 patients with active systemic lupus erythematosus. The 33 patients who had IgA nephropathy and responded to treatment demonstrated a significant decrease in CD19(+)CD5(+) B cells in the peripheral blood, peritoneal fluid, and kidney (all P < 0.01). In the three patients who had IgA nephropathy and did not respond to treatment, the frequency of CD19(+)CD5(+) B cells did not change. CD19(+)CD5(+) B cells isolated from patients with untreated IgA nephropathy expressed higher levels of IgA, produced more IFN-gamma, and were more resistant to CD95L-induced apoptosis than cells isolated from control subjects and patients with lupus; these properties reversed with effective treatment of IgA nephropathy. In conclusion, these results strongly suggest that CD19(+)CD5(+) B cells play a prominent role in the pathogenesis of primary IgA nephropathy.
学科主题	Urology & Nephrology
类目[WOS]	Urology & Nephrology
关键词[WOS]	SYSTEMIC LUPUS-ERYTHEMATOSUS ; B-1 CELLS ; AUTOIMMUNE-DISEASE ; TRANSGENIC MICE ; CHOLERA-TOXIN ; T-CELLS ; DIFFERENTIATION ; EXPRESSION ; ROLES ; CD5
收录类别	SCI
语种	英语
WOS记录号	WOS:000260588100014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1453]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	He, YL,Xiao, RJ,Ji, X,et al. CD19(+)CD5(+) B Cells in Primary IgA Nephropathy[J]. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,2008,19(11):2130-2139.
APA	He, YL.,Xiao, RJ.,Ji, X.,Jiang, YP.,Chen, L.,...&Tan, JQ.(2008).CD19(+)CD5(+) B Cells in Primary IgA Nephropathy.JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,19(11),2130-2139.
MLA	He, YL,et al."CD19(+)CD5(+) B Cells in Primary IgA Nephropathy".JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 19.11(2008):2130-2139.