Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes

	Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes
	Zhao, J; Huang, QL; Tang, YH; Guo, ZY; Qiao, ZS; Xu, GJ; Feng, YM
刊名	PROTEIN AND PEPTIDE LETTERS
	2008
卷号	15 期号:9 页码:972-979
关键词	insulin single-chain insulin folding unfolding intermediate disulfide
通讯作者	Feng, YM (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, SIBS, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,fengym@sunm.shcnc.ac.cn
英文摘要	We use the procedure established for 'disulfide stability analysis in redox system' to investigate the unfolding process of porcine insulin precursor (PIP). Six major unfolding intermediates have been captured, in which four contain two disulfides, two contain one disulfide. Based on the characterization and analysis of the intermediates an unfolding pathway has been proposed, by which the native PIP unfolded through in turn 2SS and 1SS intermediates into fully reduced form. Besides, the comparison of the intermediates captured in PIP unfolding process with those intermediates captured in its refolding process revealed that some intermediates captured during both unfolding/refolding processes of PIP have identical disulfide pairing pattern, from which we suggest that the unfolding/refolding processes of PIP share some common intermediates but flow in the opposite direction.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	BOVINE PANCREATIC RIBONUCLEASE ; DISULFIDE-FORMING PATHWAY ; SINGLE-CHAIN INSULIN ; GROWTH-FACTOR-I ; BOND FORMATION ; PEPTIDE MODEL ; PROTEIN ; VITRO ; IDENTIFICATION ; REGENERATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000259509800016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1434]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, J,Huang, QL,Tang, YH,et al. Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes[J]. PROTEIN AND PEPTIDE LETTERS,2008,15(9):972-979.
APA	Zhao, J.,Huang, QL.,Tang, YH.,Guo, ZY.,Qiao, ZS.,...&Feng, YM.(2008).Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes.PROTEIN AND PEPTIDE LETTERS,15(9),972-979.
MLA	Zhao, J,et al."Equilibrium folding of porcine insulin precursor in the presence of redox buffer: Implications for the common intermediates shared by its unfolding/refolding processes".PROTEIN AND PEPTIDE LETTERS 15.9(2008):972-979.