Localized-Statistical Quantification of Human Serum Proteome Associated with Type 2 Diabetes

	Localized-Statistical Quantification of Human Serum Proteome Associated with Type 2 Diabetes
	Li, RX; Chen, HB; Tu, K; Zhao, SL; Zhou, H; Li, SJ; Dai, J; Li, QR; Nie, S; Li, YX
刊名	PLOS ONE
	2008
卷号	3 期号:9 页码:e3224-e3224
通讯作者	Li, RX (reprint author), Chinese Acad Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China.,wpjia@yahoo.com ; zr@sibs.ac.cn ; wujr@sibs.ac.cn
英文摘要	Background: Recent advances in proteomics have shed light to discover serum proteins or peptides as biomarkers for tracking the progression of diabetes as well as understanding molecular mechanisms of the disease. Results: In this work, human serum of non-diabetic and diabetic cohorts was analyzed by proteomic approach. To analyze total 1377 high-confident serum-proteins, we developed a computing strategy called localized statistics of protein abundance distribution (LSPAD) to calculate a significant bias of a particular protein-abundance between these two cohorts. As a result, 68 proteins were found significantly over-represented in the diabetic serum (p < 0.01). In addition, a pathway-associated analysis was developed to obtain the overall pathway bias associated with type 2 diabetes, from which the significant over-representation of complement system associated with type 2 diabetes was uncovered. Moreover, an upstream activator of complement pathway, ficolin-3, was observed over-represented in the serum of type 2 diabetic patients, which was further validated with statistic significance (p=0.012) with more clinical samples. Conclusions: The developed LSPAD approach is well fit for analyzing proteomic data derived from biological complex systems such as plasma proteome. With LSPAD, we disclosed the comprehensive distribution of the proteins associated with diabetes in different abundance levels and the involvement of ficolin-related complement activation in diabetes.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000264428000007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1423]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, RX,Chen, HB,Tu, K,et al. Localized-Statistical Quantification of Human Serum Proteome Associated with Type 2 Diabetes[J]. PLOS ONE,2008,3(9):e3224-e3224.
APA	Li, RX.,Chen, HB.,Tu, K.,Zhao, SL.,Zhou, H.,...&Wu, JR.(2008).Localized-Statistical Quantification of Human Serum Proteome Associated with Type 2 Diabetes.PLOS ONE,3(9),e3224-e3224.
MLA	Li, RX,et al."Localized-Statistical Quantification of Human Serum Proteome Associated with Type 2 Diabetes".PLOS ONE 3.9(2008):e3224-e3224.