Human amniotic epithelial cells ameliorate behavioral dysfunction and reduce infarct size in the rat middle cerebral artery occlusion model

	Human amniotic epithelial cells ameliorate behavioral dysfunction and reduce infarct size in the rat middle cerebral artery occlusion model
	Liu, TJ; Wu, JC; Huang, Q; Hou, YN; Jiang, ZH; Zang, SY; Guo, LH
刊名	SHOCK
	2008
卷号	29 期号:5 页码:603-611
关键词	human amniotic epithelial cells ischemia transplantation GDNF lentiviral vector
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Genet Engn Grp, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,lhguo@sibs.ac.cn
英文摘要	Human amniotic epithelial cells (hAECs), having the characteristics of both embryonic and pluripotent stem cells, have the potential to differentiate into various cells. A good deal of research has explored the clinical therapeutic potential of hAECs; rat amniotic epithelial cells have been reported to ameliorate functional deficits after stroke in rats, likely due to neuronal differentiation and cytokine secretion by these cells. We isolated hAECs and transfected them with glial cell line-derived neurotrophic factor (GDNF) or enhanced green fluorescent protein (EGFP) gene using lentiviral vectors. These cells were then transplanted into the brains of rats subjected to a transient middle cerebral artery occlusion. The hAECs survived and migrated to the ischemic area of rats, and some of the transplanted hAECs expressed the neuronal marker MAP2 and the neuronal progenitor marker Nestin, together with the astrocyte marker glial fibrillary acidic protein, and hAEC-EGFP can significantly ameliorate behavioral dysfunction and reduce infarct volume of ischemic rats. By transfecting the cells with lentiviral vectors, GDNF can be stably overexpressed in hAECs, and hAEC-GDNF can more rapidly rescue the deficits of rats after middle cerebral artery occlusion compared with hAEC-EGFP-treated rats. Moreover, the nontransduced cells also had effects comparable to the GDNF-transduced cells on caspase-3 and lesion volume. Because hAECs are in unlimited supply, and their use is not encumbered by ethical arguments, hAECs have a great advantage for stem cell therapy. This model holds tremendous potential for development into wide use in cell-mediated gene therapy in the future.
学科主题	General & Internal Medicine; Hematology; Surgery; Cardiovascular System & Cardiology
类目[WOS]	Critical Care Medicine ; Hematology ; Surgery ; Peripheral Vascular Disease
关键词[WOS]	GENE-TRANSFER ; PARKINSONS-DISEASE ; LENTIVIRAL VECTORS ; NONDIVIDING CELLS ; TRANSGENE CARRIER ; STEM-CELLS ; IN-VIVO ; TRANSPLANTATION ; EXPRESSION ; THERAPY
收录类别	SCI
语种	英语
WOS记录号	WOS:000255210900011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1383]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, TJ,Wu, JC,Huang, Q,et al. Human amniotic epithelial cells ameliorate behavioral dysfunction and reduce infarct size in the rat middle cerebral artery occlusion model[J]. SHOCK,2008,29(5):603-611.
APA	Liu, TJ.,Wu, JC.,Huang, Q.,Hou, YN.,Jiang, ZH.,...&Guo, LH.(2008).Human amniotic epithelial cells ameliorate behavioral dysfunction and reduce infarct size in the rat middle cerebral artery occlusion model.SHOCK,29(5),603-611.
MLA	Liu, TJ,et al."Human amniotic epithelial cells ameliorate behavioral dysfunction and reduce infarct size in the rat middle cerebral artery occlusion model".SHOCK 29.5(2008):603-611.