Protein kinase A suppresses the differentiation of 3T3-L1 preadipocytes

	Protein kinase A suppresses the differentiation of 3T3-L1 preadipocytes
	Li, FQ; Wang, DM; Zhou, YR; Zhou, B; Yang, YA; Chen, HH; Song, JG
刊名	CELL RESEARCH
	2008
卷号	18 期号:2 页码:311-323
关键词	cAMP PKA adipocyte adipogenesis C/EBP PPAR
通讯作者	Song, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jgsong@sibs.ac.cn
英文摘要	cAMP and protein kinase A (PKA) are widely known as signaling molecules that are important for the induction of adipogenesis. Here we show that a strong increase in the amount of cAMP inhibits the adipogenesis of 3T3-LI fibroblast cells., Stimulation of PKA activity suppresses adipogenesis and, in contrast, inhibition of PKA activity markedly accelerates the adipogenic process. As adipogenesis progresses, there is a significant increase in the expression level of PKA regulatory subunits and a corresponding decrease in PKA activity. Moreover, treatment of 3T3-L1 cells with epidermal growth factor (EGF) stimulates PKA activity and blocks adipogenesis. Inhibition of PKA activity abolishes this suppressive effect of EGF on adipogenesis. Moreover, activation of PKA induces serine/threonine phosphorylation, reduces tyrosine phosphorylation of insulin receptor substrate 1 (IRS-1) and the association between PKA and IRS-1. Taken together, our study demonstrates that PKA has a pivotal role in the suppression of adipogenesis. cAMP at high concentrations can suppress adipogenesis through PKA activation. These findings could be important and useful for understanding the mechanisms of adipogenesis and the relevant physiological events.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	INSULIN-RECEPTOR SUBSTRATE-1 ; NUCLEOTIDE PHOSPHODIESTERASE 3B ; EPIDERMAL GROWTH-FACTOR ; TUMOR-NECROSIS-FACTOR ; ADIPOCYTE DIFFERENTIATION ; CYCLIC-AMP ; ADIPOSE-TISSUE ; ESSENTIAL REGULATOR ; GENE-EXPRESSION ; PPAR-GAMMA
收录类别	SCI
语种	英语
WOS记录号	WOS:000254024700012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1341]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, FQ,Wang, DM,Zhou, YR,et al. Protein kinase A suppresses the differentiation of 3T3-L1 preadipocytes[J]. CELL RESEARCH,2008,18(2):311-323.
APA	Li, FQ.,Wang, DM.,Zhou, YR.,Zhou, B.,Yang, YA.,...&Song, JG.(2008).Protein kinase A suppresses the differentiation of 3T3-L1 preadipocytes.CELL RESEARCH,18(2),311-323.
MLA	Li, FQ,et al."Protein kinase A suppresses the differentiation of 3T3-L1 preadipocytes".CELL RESEARCH 18.2(2008):311-323.