Identification of zebrafish LPTS: A gene with similarities to human LPTS/PinX1 that inhibits telomerase activity

	Identification of zebrafish LPTS: A gene with similarities to human LPTS/PinX1 that inhibits telomerase activity
	Sun, CF; Wu, ZL; Jia, FJ; Wang, YF; Li, TP; Zhao, MJ
刊名	GENE
	2008
卷号	420 期号:1 页码:90-98
关键词	cloning expression nucleolus telomere
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn
英文摘要	Human LPTS/PinX1 is a newly identified telomerase inhibitory protein. Overexpression of the LPTS/PinX1 gene suppresses telomerase activity, results in shortened telomeres. To investigate the role of the LPTS gene in zebrafish, we cloned the homologous gene, zLPTS, which encodes a protein of 355 amino acids. Sequence analysis revealed that, like human LPTS/PinX1, the zLPTS protein has a conserved G-patch domain at its N-terminus and a lysine-rich domain at its C-terminus. Bioinformatics analysis showed the evolutionary conservation of zLPTS. Using RT-PCR and northern blot, we found that zLPTS was expressed in all zebrafish tissues with higher level in ovary, and in all embryonic developmental stages examined. Whole mount in situ hybridization revealed that zLPTS was expressed in all regions of early developmental embryos. The subcellular localization of zLPTS protein was showed in the nucleolus and telomeres. We also cloned the gene for zebrafish Telomerase Reverse Transcriptase (zTERT), a catalytic subunit of telomerase, and demonstrated that zLPTS protein can interact with zTERT through the TR-binding domain of zTERT. Further, we verified that zLPTS could inhibit telomerase activities in zebrafish embryos and human cancer cell line by TRAP assay. Our results clearly demonstrate that zLPTS is ubiquitously expressed in tissues and embryos and plays a function of inhibiting telomerase activity. This study may provide a useful system for further investigating the mechanism of telomere length regulation. (C) 2008 Elsevier B.V. All rights reserved.
学科主题	Genetics & Heredity
类目[WOS]	Genetics & Heredity
关键词[WOS]	PUTATIVE-TUMOR-SUPPRESSOR ; REVERSE-TRANSCRIPTASE ; CATALYTIC SUBUNIT ; PROTEIN ; RNA ; LENGTH ; HTERT ; PINX1 ; EXPRESSION ; KINASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000258249400012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1317]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Sun, CF,Wu, ZL,Jia, FJ,et al. Identification of zebrafish LPTS: A gene with similarities to human LPTS/PinX1 that inhibits telomerase activity[J]. GENE,2008,420(1):90-98.
APA	Sun, CF,Wu, ZL,Jia, FJ,Wang, YF,Li, TP,&Zhao, MJ.(2008).Identification of zebrafish LPTS: A gene with similarities to human LPTS/PinX1 that inhibits telomerase activity.GENE,420(1),90-98.
MLA	Sun, CF,et al."Identification of zebrafish LPTS: A gene with similarities to human LPTS/PinX1 that inhibits telomerase activity".GENE 420.1(2008):90-98.