Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice

	Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice
	Zhang, JF; Gui, YP; Yuan, T; Bian, CD; Guo, LH
刊名	SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
	2009
卷号	55 期号:1 页码:175-180
关键词	GAT1 epididymis reproduction sperm testes transgenic mice
通讯作者	Zhang, JF (reprint author), Tongji Univ, Dept Urol, Tongji Hosp, 389 Xincun Rd, Shanghai 200065, Peoples R China.,jinfuzhang@yahoo.com.cn
英文摘要	The present study was carried out to identify GABA (gamma-aminobutyric acid) transport protein I (GAT1) in male reproductive organs and to study the effect of GAT1 overexpression on the male reproductive system in GAT1 transgenic mice (TG). Expression and location of GAT1 in testes, epididymis, and sperm of wild-type (WT) mice were identified by immunohistochemistry and western-blot. Histological changes of testes, epididymis, and sperm of transgenic mice overexpressing GAT1 were detected by immunofluorenscent staining and haematoxylin and ecisin (HE) staining. GAT1 expression was detected in the testes, epididymis, and sperm of non-transgenic mice. Vacuolization and deformity of spermatogenic cells were observed in the transgenic mice, but the epididymis was unremarkable. Immunofluorenscent staining showed that the number of diastrophic and decapitated sperm increased significantly in transgenic mice to 46.9% from 7.3% in nontransgenic mice. These results suggest that abnormal expression of GAT1 could result in spermiogenesis function injury, sperm paramorphia and dysgenesis.
学科主题	Endocrinology & Metabolism; Reproductive Biology
类目[WOS]	Andrology ; Reproductive Biology
关键词[WOS]	GAMMA-AMINOBUTYRIC-ACID ; SPERM ACROSOME REACTION ; GABA(A) RECEPTOR ; RAT TESTIS ; TRANSPORTER ; BRAIN ; IDENTIFICATION ; PROGESTERONE
收录类别	SCI
语种	英语
WOS记录号	WOS:000272975100001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1306]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, JF,Gui, YP,Yuan, T,et al. Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice[J]. SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE,2009,55(1):175-180.
APA	Zhang, JF,Gui, YP,Yuan, T,Bian, CD,&Guo, LH.(2009).Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice.SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE,55(1),175-180.
MLA	Zhang, JF,et al."Expression of GAT1 in Male Reproductive System and its Effects on Reproduction in Mice".SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE 55.1(2009):175-180.