Requirement of Myosin Vb.Rab11a.Rab11-FIP2 Complex in Cholesterol-regulated Translocation of NPC1L1 to the Cell Surface

	Requirement of Myosin Vb.Rab11a.Rab11-FIP2 Complex in Cholesterol-regulated Translocation of NPC1L1 to the Cell Surface
	Chu, BB; Ge, L; Xie, C; Zhao, Y; Miao, HH; Wang, J; Li, BL; Song, BL
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2009
卷号	284 期号:33 页码:22481-22490
通讯作者	Song, BL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,blsong@sibs.ac.cn
英文摘要	Niemann-Pick C1-like 1 (NPC1L1) plays a critical role in the enterohepatic absorption of free cholesterol. Cellular cholesterol depletion induces the transport of NPC1L1 from the endocytic recycling compartment to the plasma membrane (PM), and cholesterol replenishment causes the internalization of NPC1L1 together with cholesterol via clathrin-mediated endocytosis. Although NPC1L1 has been characterized, the other proteins involved in cholesterol absorption and the endocytic recycling of NPC1L1 are largely unknown. Most of the vesicular trafficking events are dependent on the cytoskeleton and motor proteins. Here, we investigated the roles of the microfilament and microfilament-associated triple complex composed of myosin Vb, Rab11a, and Rab11-FIP2 in the transport of NPC1L1 from the endocytic recycling compartment to the PM. Interfering with the dynamics of the microfilament by pharmacological treatment delayed the transport of NPC1L1 to the cell surface. Meanwhile, inactivation of any component of the myosin Vb.Rab11a.Rab11-FIP2 triple complex inhibited the export of NPC1L1. Expression of the dominant-negative mutants of myosin Vb, Rab11a, or Rab11-FIP2 decreased the cellular cholesterol uptake by blocking the transport of NPC1L1 to the PM. These results suggest that the efficient transport of NPC1L1 to the PM is dependent on the microfilament-associated myosin Vb.Rab11a.Rab11-FIP2 triple complex.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	HMG-COA REDUCTASE ; EPITHELIAL-CELLS ; RECYCLING ENDOSOMES ; VB ; ACTIN ; CYTOSKELETON ; TRAFFICKING ; TRANSFERRIN ; ABSORPTION ; TRANSPORT
收录类别	SCI
语种	英语
WOS记录号	WOS:000268783700069
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1290]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chu, BB,Ge, L,Xie, C,et al. Requirement of Myosin Vb.Rab11a.Rab11-FIP2 Complex in Cholesterol-regulated Translocation of NPC1L1 to the Cell Surface[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(33):22481-22490.
APA	Chu, BB.,Ge, L.,Xie, C.,Zhao, Y.,Miao, HH.,...&Song, BL.(2009).Requirement of Myosin Vb.Rab11a.Rab11-FIP2 Complex in Cholesterol-regulated Translocation of NPC1L1 to the Cell Surface.JOURNAL OF BIOLOGICAL CHEMISTRY,284(33),22481-22490.
MLA	Chu, BB,et al."Requirement of Myosin Vb.Rab11a.Rab11-FIP2 Complex in Cholesterol-regulated Translocation of NPC1L1 to the Cell Surface".JOURNAL OF BIOLOGICAL CHEMISTRY 284.33(2009):22481-22490.