B23 interacts with PES1 and is involved in nucleolar localization of PES1

	B23 interacts with PES1 and is involved in nucleolar localization of PES1
	Zhang, JH; Yang, Y; Wu, JR
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2009
卷号	41 期号:12 页码:991-997
关键词	B23 PES1 cell nucleolus
通讯作者	Wu, JR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol,State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,wujr@sibs.ac.cn
英文摘要	PES1, the human homolog of zebrafish pescadillo, is a nucleolar protein that is essential for cell proliferation. We report herein that a nucleolar marker protein B23 physically interacts with PES1 and is involved in the nucleolar localization of PES1. In vivo interaction between B23 and PES1 was verified by co-immunoprecipitation of endogenous B23 and PES1 proteins, and they showed cellular co-localizations under both normal and actinomycin D-induced stress conditions. Furthermore, we mapped their interaction domains via in vitro pull-down assays. When B23 was knocked down by RNA interference, there appeared an increased nucleoplasmic distribution of PES1. Our results support a previous hypothesis that B23 might be a nucleolar hub protein for protein targeting to the nucleolus, and shed light on the nucleolar localization mechanism of PES1. The physical interaction between B23 and PES1 implies that they may participate in ribosome biogenesis in a protein complex.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	VIRUS TYPE-1 REV ; RIBOSOME BIOGENESIS ; CELL-PROLIFERATION ; RESPONSE ELEMENT ; PEBOW-COMPLEX ; PROTEIN B23 ; NUCLEOPHOSMIN ; PESCADILLO ; CANCER ; TRANSLOCATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000272463800003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1261]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, JH,Yang, Y,Wu, JR. B23 interacts with PES1 and is involved in nucleolar localization of PES1[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(12):991-997.
APA	Zhang, JH,Yang, Y,&Wu, JR.(2009).B23 interacts with PES1 and is involved in nucleolar localization of PES1.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(12),991-997.
MLA	Zhang, JH,et al."B23 interacts with PES1 and is involved in nucleolar localization of PES1".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.12(2009):991-997.