Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation

	Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation
	Zhou, J; Zhou, BO; Lenzmeier, BA; Zhou, JQ
刊名	NUCLEIC ACIDS RESEARCH
	2009
卷号	37 期号:11 页码:3699-3713
通讯作者	Zhou, J (reprint author), Chinese Acad Sci, Chinese Acad Sci, Inst Biochem & Cell Biol, Inst Biol Sci, 320 Yue Yang Rd, Shanghai 2, Peoples R China.,jqzhou@sibs.ac.cn
英文摘要	In the eukaryotic genome, transcriptionally silent chromatin tends to propagate along a chromosome and encroach upon adjacent active chromatin. The silencing machinery can be stopped by chromatin boundary elements. We performed a screen in Saccharomyces cerevisiae for proteins that may contribute to the establishment of a chromatin boundary. We found that disruption of histone deacetylase Rpd3p results in defective boundary activity, leading to a Sir-dependent local propagation of transcriptional repression. In rpd3 cells, the amount of Sir2p that was normally found in the nucleolus decreased and the amount of Sir2p found at telomeres and at HM and its adjacent loci increased, leading to an extension of silent chromatin in those areas. In addition, Rpd3p interacted directly with chromatin at boundary regions to deacetylate histone H4 at lysine 5 and at lysine 12. Either the mutation of histone H4 at lysine 5 or a decrease in the histone acetyltransferase (HAT) activity of Esa1p abrogated the silencing phenotype associated with rpd3 mutation, suggesting a novel role for the H4 amino terminus in Rpd3p-mediated heterochromatin boundary regulation. Together, these data provide insight into the molecular mechanisms for the anti-silencing functions of Rpd3p during the formation of heterochromatin boundaries.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; GENOME-WIDE ; HETEROCHROMATIN BOUNDARIES ; REPRESSED CHROMATIN ; GENE DELETION ; VARIANT H2A.Z ; YEAST RDNA ; IN-VIVO ; COMPLEX ; ACETYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000267441800027
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1258]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, J,Zhou, BO,Lenzmeier, BA,et al. Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation[J]. NUCLEIC ACIDS RESEARCH,2009,37(11):3699-3713.
APA	Zhou, J,Zhou, BO,Lenzmeier, BA,&Zhou, JQ.(2009).Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation.NUCLEIC ACIDS RESEARCH,37(11),3699-3713.
MLA	Zhou, J,et al."Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation".NUCLEIC ACIDS RESEARCH 37.11(2009):3699-3713.