Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport

	Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport
	Zhang, QG; Wang, FB; Cao, JL; Shen, YD; Huang, QP; Bao, L; Zhu, X
刊名	TRAFFIC
	2009
卷号	10 期号:9 页码:1337-1349
关键词	axon dynein endosome lysosome mitochondrion neuron Nudel vesicle transport
通讯作者	Zhu, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn
英文摘要	Axonal transport is critical for neuronal function and survival. Cytoplasmic dynein and its accessory complex dynactin form a microtubule minus end-directed motor in charge of retrograde transport. In this study, we show that Nudel, a dynein regulator, was highly expressed in dorsal root ganglion (DRG) neurons. Microinjection of anti-Nudel antibody into cultured DRG neurons abolished retrograde transport of membranous organelles in the axon and led to dispersions of Golgi cisternae in the soma. As a result, lysosomes, which are normally enriched in the soma, moved persistently into and thus accumulated in axons. Endo-lysosome formation was also markedly delayed. As anterograde motility of mitochondria was not inhibited, the antibody apparently did not abolish retrograde transport by destructing axonal microtubule tracks. Similar results were obtained by microinjecting N-terminal Nudel, anti-dynein antibody or a p150(Glued) mutant capable of abrogating the dynein-dynactin association. These results indicate a critical role of Nudel in dynein-mediated axonal transport. Moreover, the effects of dynein on endolysosome formation and regional sequestration of lysosomes may contribute to defects in the endocytic pathway seen in neurons of patients or animals with malfunction of dynein.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	MOTOR-NEURON DISEASE ; CYTOPLASMIC DYNEIN ; RETROGRADE TRANSPORT ; HIPPOCAMPAL-NEURONS ; DYNACTIN COMPLEX ; MUTANT DYNACTIN ; MIGRATING CELLS ; GROWTH CONE ; PROTEIN ; MICROTUBULES
收录类别	SCI
语种	英语
WOS记录号	WOS:000268794100013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1237]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, QG,Wang, FB,Cao, JL,et al. Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport[J]. TRAFFIC,2009,10(9):1337-1349.
APA	Zhang, QG.,Wang, FB.,Cao, JL.,Shen, YD.,Huang, QP.,...&Zhu, X.(2009).Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport.TRAFFIC,10(9),1337-1349.
MLA	Zhang, QG,et al."Nudel Promotes Axonal Lysosome Clearance and Endo-lysosome Formation via Dynein-Mediated Transport".TRAFFIC 10.9(2009):1337-1349.