Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1

	Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1
	Du, D; Pedersen, E; Wang, ZP; Karlsson, R; Chen, ZJ; Wu, XW; Brakebusch, C
刊名	EXPERIMENTAL CELL RESEARCH
	2009
卷号	315 期号:8 页码:1480-1489
关键词	Cdc42 Cell junction maturation Keratinocytes
通讯作者	Brakebusch, C (reprint author), Univ Copenhagen, Inst Biomed, BRIC, Ole Maaloes Vej 5, DK-2200 Copenhagen, Denmark.,cord.brakebusch@bric.dk
英文摘要	Cell-cell contacts are crucial for the integrity of all tissues. Contrasting reports have been published about the role of Cdc42 in epithelial cell-cell contacts in vitro. In keratinocytes, it was suggested that Rac1 and not Cdc42 is crucial for the formation of mature epithelial junctions, based on dominant negative inhibition experiments. Deletion of the Cdc42 gene in keratinocytes in vivo slowly impaired the maintenance of cell-cell contacts by an increased degradation of beta-catenin. Whether Cdc42 is required for the formation of mature junctions was not tested. We show now that Cdc42-deficient immortalized and primary keratinocytes form only punctate primordial cell contacts in vitro, which cannot mature into belt-like junctions. This defect was independent of enhanced degradation of beta-catenin, but correlated to an impaired activation and localization of aPKC zeta in the Cdc42-null keratinocytes. Inhibition of aPKC zeta by the inhibitor Go6983 reproduced the phenotype, suggesting that decreased activation of aPKC zeta was sufficient to explain the defective junctional maturation. In the absence of Cdc42, Rac1 activation was strongly decreased, indicating that Cdc42 is upstream of Rac1 activation. These data reveal that Cdc42 is crucial for the formation of mature epithelial cell junctions between keratinocytes by regulating activation of aPKC zeta. (C) 2008 Elsevier Inc. All rights reserved.
学科主题	Oncology ; Cell Biology
类目[WOS]	Oncology ; Cell Biology
关键词[WOS]	MDCK CELLS ; EPITHELIAL POLARITY ; TIGHT ; APKC ; ACTIVATION ; ADHESION ; COMPLEX ; PROTEIN ; DIFFERENTIATION ; ESTABLISHMENT
收录类别	SCI
语种	英语
WOS记录号	WOS:000265438300013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1230]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Du, D,Pedersen, E,Wang, ZP,et al. Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1[J]. EXPERIMENTAL CELL RESEARCH,2009,315(8):1480-1489.
APA	Du, D.,Pedersen, E.,Wang, ZP.,Karlsson, R.,Chen, ZJ.,...&Brakebusch, C.(2009).Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1.EXPERIMENTAL CELL RESEARCH,315(8),1480-1489.
MLA	Du, D,et al."Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1".EXPERIMENTAL CELL RESEARCH 315.8(2009):1480-1489.