NF-kappa B Transcription Factor p50 Critically Regulates Tissue Factor in Deep Vein Thrombosis

	NF-kappa B Transcription Factor p50 Critically Regulates Tissue Factor in Deep Vein Thrombosis
	Li, YD; Ye, BQ; Zheng, SX; Wang, JT; Wang, JG; Chen, M; Liu, JG; Pei, XH; Wang, LJ; Lin, ZX
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2009
卷号	284 期号:7 页码:4473-4483
通讯作者	Geng, JG (reprint author), MMC 480,420 Delaware St SE, Minneapolis, MN 55455 USA.,genglab@gmail.com
英文摘要	NF-kappa B transcription factors regulate the expression of tissue factor (TF), a principal initiator of the coagulation cascade. Dominant among them is the p50/p65 heterodimer. Here we report that Andrographolide (Andro; a p50 inhibitor) and genetic deletion of p50 attenuated TF activity in stimulated endothelial cells and monocytes/macrophages. Results of the electrophoretic mobility "supershift" assay and chromatin immunoprecipitation demonstrated the direct interaction of the p50/p65 heterodimer with the NF-kappa B site of the human TF promoter. Andro-treated and p50 null mice both exhibited blunted TF expression and reduced venous thrombosis, which were recapitulated by an anti-murine TF antibody in vivo. Our findings thus indicate that regulation of TF by NF-kappa B transcription factor p50 is essential for the pathogenesis of deep vein thrombosis and suggest that specific inhibitors of p50, such as Andro, may be therapeutically valuable for preventing and perhaps treating venous thrombosis.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	P-SELECTIN ; CELL-ADHESION ; VENOUS THROMBOSIS ; FACTOR GENE ; ENDOTHELIAL-CELLS ; MONOCYTIC CELLS ; DEFICIENT MICE ; CANCER GROWTH ; BONE-MARROW ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000263134400047
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1222]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, YD,Ye, BQ,Zheng, SX,et al. NF-kappa B Transcription Factor p50 Critically Regulates Tissue Factor in Deep Vein Thrombosis[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(7):4473-4483.
APA	Li, YD.,Ye, BQ.,Zheng, SX.,Wang, JT.,Wang, JG.,...&Geng, JG.(2009).NF-kappa B Transcription Factor p50 Critically Regulates Tissue Factor in Deep Vein Thrombosis.JOURNAL OF BIOLOGICAL CHEMISTRY,284(7),4473-4483.
MLA	Li, YD,et al."NF-kappa B Transcription Factor p50 Critically Regulates Tissue Factor in Deep Vein Thrombosis".JOURNAL OF BIOLOGICAL CHEMISTRY 284.7(2009):4473-4483.