CIN85 associates with endosomal membrane and binds phosphatidic acid

	CIN85 associates with endosomal membrane and binds phosphatidic acid
	Zhang, J; Zheng, XD; Yang, X; Liao, K
刊名	CELL RESEARCH
	2009
卷号	19 期号:6 页码:733-746
关键词	CIN85 the coiled-coil domain phosphatidic acid EGFR endocytosis ESCRT assembly
通讯作者	Liao, K (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn
英文摘要	CIN85 (Cbl-interacting protein of 85 kDa) is an important molecule involved in receptor tyrosine kinase endocytosis. Here we report that through its positively charged C-terminus, CIN85 associates with a fusogenic lipid - phosphatidic acid. Its coiled-coil domain plays an important role in mediating this protein-lipid interaction. Deletion of the coiled-coil domain results in loss of membrane association, and reduced interaction with c-cbl, finally causing the blockage of epidermal growth factor receptor downregulation. In addition, a significant portion of CIN85 is located on the endosomal compartment and is related to endocytic cargo sorting, characterized by CIN85's localization on the "E class" compartment and EGF degradation blockage in CIN85 knockdown cells. Taken together, our results suggest that CIN85 may function as a scaffold molecule in both the internalization and endocytic cargo sorting processes through its association with the endosomal membrane.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	RECEPTOR DOWN-REGULATION ; GROWTH-FACTOR RECEPTORS ; PHOSPHOLIPASE-D ; TYROSINE KINASES ; MAMMALIAN-CELLS ; EGF RECEPTORS ; C-CBL ; PROTEIN ; COMPLEX ; TRAFFICKING
收录类别	SCI
语种	英语
WOS记录号	WOS:000266528000007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1207]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, J,Zheng, XD,Yang, X,et al. CIN85 associates with endosomal membrane and binds phosphatidic acid[J]. CELL RESEARCH,2009,19(6):733-746.
APA	Zhang, J,Zheng, XD,Yang, X,&Liao, K.(2009).CIN85 associates with endosomal membrane and binds phosphatidic acid.CELL RESEARCH,19(6),733-746.
MLA	Zhang, J,et al."CIN85 associates with endosomal membrane and binds phosphatidic acid".CELL RESEARCH 19.6(2009):733-746.