Comparative Proteomic Analysis of Proteins Involved in Cell Aggregation during Neural Differentiation of P19 Mouse Embryonic Carcinoma Cells

	Comparative Proteomic Analysis of Proteins Involved in Cell Aggregation during Neural Differentiation of P19 Mouse Embryonic Carcinoma Cells
	Gao, X; Tian, HY; Liu, L; Yu, ML; Jing, NH; Zhao, FK
刊名	JOURNAL OF PROTEOME RESEARCH
	2009
卷号	8 期号:4 页码:1765-1781
关键词	P19 cell Cell aggregation Neural differentiation Comparative proteomics NDRG1 RNA interference
通讯作者	Jing, NH (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,njing@sibs.ac.cn ; fkzhao@sibs.ac.cn
英文摘要	Cell-cell interactions play a crucial role during embryogenesis and are enhanced during cell aggregation. P19 mouse embryonic carcinoma cells can differentiate into neural cells by the addition of retinoic acid (RA) or by overexpression of the Wnt1 gene, with both processes dependent on cell aggregation. To identify molecules involved in the cell aggregation process, two-dimensional gel electrophoresis (2DE) was used to establish the cell aggregation-associated protein profiles. MALDI-TOF/TOF was used to identify 71 protein spots with differential expression patterns. Among these spots, 54 were differentially expressed in both P19 and Wnt1-overexpressing P19 (Wnt1/P19) cell aggregates, with 42 proteins up-regulated and 12 proteins down-regulated. The other 17 spots were differentially expressed only in Wnt1/P19 cells. The expression patterns of 5 cell aggregation-associated proteins, N-myc downstream-regulated gene 1 (NDRG1), 14-3-3 epsilon, 14-3-3 gamma, acid calponin and cell division control protein 2 homologue (Cdc2), were confirmed by immunoblot and RT-PCR. To further investigate the relationship between cell aggregation and neural differentiation, NDRG1 expression was inhibited by RNA interference during P19 cell aggregation. Silencing of NDRG1 reduced the size of cell aggregates and the expression of N-cadherin, and it also impaired the RA-induced P19 cell neural differentiation. In conclusion, this study provides new clues for the possible mechanism underlying cell aggregation during pluripotent stem cell neural differentiation.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	STEM-CELLS ; IN-VITRO ; NEURONAL DIFFERENTIATION ; RETINOIC-ACID ; GENE-EXPRESSION ; N-CADHERIN ; IDENTIFICATION ; GASTRULATION ; EMBRYOGENESIS ; INDUCTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000264928200016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1180]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gao, X,Tian, HY,Liu, L,et al. Comparative Proteomic Analysis of Proteins Involved in Cell Aggregation during Neural Differentiation of P19 Mouse Embryonic Carcinoma Cells[J]. JOURNAL OF PROTEOME RESEARCH,2009,8(4):1765-1781.
APA	Gao, X,Tian, HY,Liu, L,Yu, ML,Jing, NH,&Zhao, FK.(2009).Comparative Proteomic Analysis of Proteins Involved in Cell Aggregation during Neural Differentiation of P19 Mouse Embryonic Carcinoma Cells.JOURNAL OF PROTEOME RESEARCH,8(4),1765-1781.
MLA	Gao, X,et al."Comparative Proteomic Analysis of Proteins Involved in Cell Aggregation during Neural Differentiation of P19 Mouse Embryonic Carcinoma Cells".JOURNAL OF PROTEOME RESEARCH 8.4(2009):1765-1781.